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- W2024492382 abstract "Isoprostanes are a family of prostaglandin isomers produced from oxidation of polyunsaturated fatty acids through a non-enzymatic free radical-catalyzed mechanism. Quantification of F2-isoprostanes (F2-IsoPs) provides a good index of oxidative stress and allows non-invasive assessment of lipid peroxidation in vivo. Since “interferences peaks” at m/z 573 co-elute with d4-15-F2t-IsoP preferentially used, we propose a new GC–NICI-MS approach to quantify urinary F2-IsoPs by using 4(RS)-F4t-neuroprostane as the internal standard. This method was applied to quantify urinary F2-IsoPs excretion in healthy volunteers and polytraumatized patients. Our results showed a significant increase (p < 0.0001) in urinary F2-IsoPs in polytraumatized patients compared with healthy volunteers (4.73 ± 2.75 ng/mg vs. 0.811 ± 0.359 ng/mg creatinine)." @default.
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- W2024492382 date "2008-09-01" @default.
- W2024492382 modified "2023-10-16" @default.
- W2024492382 title "Quantification of urinary F2-isoprostanes with 4(RS)-F4t-neuroprostane as an internal standard using gas chromatography–mass spectrometry" @default.
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- W2024492382 doi "https://doi.org/10.1016/j.jchromb.2008.07.037" @default.
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