Matches in SemOpenAlex for { <https://semopenalex.org/work/W2024499862> ?p ?o ?g. }
- W2024499862 endingPage "112" @default.
- W2024499862 startingPage "103" @default.
- W2024499862 abstract "It is widely accepted that glycine transporters of the GLYT1 type are situated on astrocytes whereas GLYT2 are present on glycinergic neuronal terminals where they mediate glycine uptake. We here used purified preparations of mouse spinal cord nerve terminals (synaptosomes) and of astrocyte-derived subcellular particles (gliosomes) to characterize functionally and morphologically the glial versus neuronal distribution of GLYT1 and GLYT2. Both gliosomes and synaptosomes accumulated [3H]GABA through GAT1 transporters and, when exposed to glycine in superfusion conditions, they released the radioactive amino acid not in a receptor-dependent manner, but as a consequence of glycine penetration through selective transporters. The glycine-evoked release of [3H]GABA was exocytotic from synaptosomes but GAT1 carrier-mediated from gliosomes. Based on the sensitivity of the glycine effects to selective GLYT1 and GLYT2 blockers, the two transporters contributed equally to evoke [3H]GABA release from GABAergic synaptosomes; even more surprising, the 'neuronal' GLYT2 contributed more efficiently than the 'glial' GLYT1 to mediate the glycine effect in [3H]GABA releasing gliosomes. These functional results were largely confirmed by confocal microscopy analysis showing co-expression of GAT1 and GLYT2 in GFAP-positive gliosomes and of GAT1 and GLYT1 in MAP2-positive synaptosomes. To conclude, functional GLYT1 are present on neuronal axon terminals and functional GLYT2 are expressed on astrocytes, indicating not complete selectivity of glycine transporters in their glial versus neuronal localization in the spinal cord." @default.
- W2024499862 created "2016-06-24" @default.
- W2024499862 creator A5015365959 @default.
- W2024499862 creator A5015779278 @default.
- W2024499862 creator A5022450636 @default.
- W2024499862 creator A5037612577 @default.
- W2024499862 creator A5040887321 @default.
- W2024499862 creator A5048232498 @default.
- W2024499862 creator A5051513948 @default.
- W2024499862 creator A5076444958 @default.
- W2024499862 date "2008-01-01" @default.
- W2024499862 modified "2023-10-06" @default.
- W2024499862 title "Functional expression of release-regulating glycine transporters GLYT1 on GABAergic neurons and GLYT2 on astrocytes in mouse spinal cord" @default.
- W2024499862 cites W1534978388 @default.
- W2024499862 cites W1600455132 @default.
- W2024499862 cites W1852646540 @default.
- W2024499862 cites W1862870409 @default.
- W2024499862 cites W1911169303 @default.
- W2024499862 cites W1938064199 @default.
- W2024499862 cites W1970694301 @default.
- W2024499862 cites W1971264177 @default.
- W2024499862 cites W1972136725 @default.
- W2024499862 cites W1972326540 @default.
- W2024499862 cites W1980506971 @default.
- W2024499862 cites W1990449778 @default.
- W2024499862 cites W1992590985 @default.
- W2024499862 cites W1997118069 @default.
- W2024499862 cites W1999061814 @default.
- W2024499862 cites W1999612489 @default.
- W2024499862 cites W2002762051 @default.
- W2024499862 cites W2005430966 @default.
- W2024499862 cites W2009473514 @default.
- W2024499862 cites W2016939813 @default.
- W2024499862 cites W2020986366 @default.
- W2024499862 cites W2022050193 @default.
- W2024499862 cites W2026348045 @default.
- W2024499862 cites W2042717783 @default.
- W2024499862 cites W2045612056 @default.
- W2024499862 cites W2049594737 @default.
- W2024499862 cites W2052148365 @default.
- W2024499862 cites W2060224220 @default.
- W2024499862 cites W2060890290 @default.
- W2024499862 cites W2068813862 @default.
- W2024499862 cites W2069049686 @default.
- W2024499862 cites W2072465203 @default.
- W2024499862 cites W2074382485 @default.
- W2024499862 cites W2084414231 @default.
- W2024499862 cites W2084474788 @default.
- W2024499862 cites W2086212503 @default.
- W2024499862 cites W2107633785 @default.
- W2024499862 cites W2109226288 @default.
- W2024499862 cites W2122244180 @default.
- W2024499862 cites W2125782175 @default.
- W2024499862 cites W2134765462 @default.
- W2024499862 cites W2147137091 @default.
- W2024499862 cites W2161917727 @default.
- W2024499862 doi "https://doi.org/10.1016/j.neuint.2007.04.027" @default.
- W2024499862 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17597258" @default.
- W2024499862 hasPublicationYear "2008" @default.
- W2024499862 type Work @default.
- W2024499862 sameAs 2024499862 @default.
- W2024499862 citedByCount "51" @default.
- W2024499862 countsByYear W20244998622012 @default.
- W2024499862 countsByYear W20244998622013 @default.
- W2024499862 countsByYear W20244998622014 @default.
- W2024499862 countsByYear W20244998622015 @default.
- W2024499862 countsByYear W20244998622016 @default.
- W2024499862 countsByYear W20244998622017 @default.
- W2024499862 countsByYear W20244998622018 @default.
- W2024499862 countsByYear W20244998622019 @default.
- W2024499862 countsByYear W20244998622021 @default.
- W2024499862 countsByYear W20244998622022 @default.
- W2024499862 crossrefType "journal-article" @default.
- W2024499862 hasAuthorship W2024499862A5015365959 @default.
- W2024499862 hasAuthorship W2024499862A5015779278 @default.
- W2024499862 hasAuthorship W2024499862A5022450636 @default.
- W2024499862 hasAuthorship W2024499862A5037612577 @default.
- W2024499862 hasAuthorship W2024499862A5040887321 @default.
- W2024499862 hasAuthorship W2024499862A5048232498 @default.
- W2024499862 hasAuthorship W2024499862A5051513948 @default.
- W2024499862 hasAuthorship W2024499862A5076444958 @default.
- W2024499862 hasConcept C104317684 @default.
- W2024499862 hasConcept C149011108 @default.
- W2024499862 hasConcept C169760540 @default.
- W2024499862 hasConcept C170493617 @default.
- W2024499862 hasConcept C185592680 @default.
- W2024499862 hasConcept C2777542381 @default.
- W2024499862 hasConcept C2777725853 @default.
- W2024499862 hasConcept C2777756961 @default.
- W2024499862 hasConcept C2778921374 @default.
- W2024499862 hasConcept C2779046065 @default.
- W2024499862 hasConcept C2779177108 @default.
- W2024499862 hasConcept C2780775167 @default.
- W2024499862 hasConcept C35599011 @default.
- W2024499862 hasConcept C515207424 @default.
- W2024499862 hasConcept C529278444 @default.
- W2024499862 hasConcept C55493867 @default.
- W2024499862 hasConcept C86803240 @default.