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- W2024550544 abstract "To decide whether the change in the quaternary state of unligated hemoglobin affects the electronic and structural properties of the heme group unligated hemoglobins modified with bis‐( N ‐maleimidomethyl)ether and 2, 2′‐dicarboxy‐4, 4′‐diiodoacetamidoazobenzol, which are in the high‐affinity form, have been compared with unligated normal deoxyhemoglobin A by absorption spectroscopy and Raman scattering. The absorption spectra show no difference in the frequency of the bands in the visible and near ultraviolet region but differences were found in peak height and shape of the Soret band. The Raman resonance spectra recorded between 300 and 1700 cm −1 show no difference at all. These spectral observations indicate that the change of the quaternary structure of unligated hemoglobin slightly influences the excited electronic states of the heme but does not alter the electronic ground state. Along with other spectroscopic evidence from Mössbauer and nuclear magnetic resonance spectroscopy this suggests that only a minor fraction of the free energy difference of ligand binding in the two quaternary states may originate from differences in the electronic properties of the heme in unligated hemoglobin." @default.
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- W2024550544 date "1974-02-01" @default.
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- W2024550544 title "Raman Study on the Two Quaternary States of Unligated Hemoglobin" @default.
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- W2024550544 doi "https://doi.org/10.1111/j.1432-1033.1974.tb03288.x" @default.
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