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- W2024557832 abstract "A new highly sensitive amperometric method for the detection of organophosphorus compounds has been developed. The method is based on a ferophthalocyanine chemically modified carbon paste electrode coupled with acetylcholinesterase and choline oxidase co-immobilized onto the surface of a dialysis membrane. The activity of cholinesterase is non-competitively inhibited in the presence of pesticides. The highest sensitivity to inhibitors was found for a membrane containing low enzyme loading and this was subsequently used for the construction of an amperometric biosensor for pesticides. Analyses were done using acetylcholine as substrate; choline produced by hydrolysis in the enzymatic layer was oxidized by choline-oxidase and subsequently H2O2 produced was electrochemically detected at +0.35 V vs. Ag/AgCl. The decrease of substrate steady-state current caused by the addition of pesticide was used for evaluation. With this approach, up to 10−10 M of paraoxon and carbofuran can be detected." @default.
- W2024557832 created "2016-06-24" @default.
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- W2024557832 date "2003-03-01" @default.
- W2024557832 modified "2023-09-23" @default.
- W2024557832 title "Detection of pesticides using an amperometric biosensor based on ferophthalocyanine chemically modified carbon paste electrode and immobilized bienzymatic system" @default.
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- W2024557832 doi "https://doi.org/10.1016/s0956-5663(02)00173-2" @default.
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