Matches in SemOpenAlex for { <https://semopenalex.org/work/W2024558947> ?p ?o ?g. }
- W2024558947 endingPage "2265" @default.
- W2024558947 startingPage "2255" @default.
- W2024558947 abstract "EspC is a non-locus of enterocyte effacement (LEE)-encoded autotransporter produced by enteropathogenic Escherichia coli (EPEC) that is secreted to the extracellular milieu by a type V secretion system and then translocated into epithelial cells by the type III secretion system. Here, we show that this efficient EspC delivery into the cell leads to a cytopathic effect characterized by cell rounding and cell detachment. Thus, EspC is the main protein involved in epithelial cell cytotoxicity detected during EPEC adhesion and pedestal formation assays. The cell detachment phenotype is triggered by cytoskeletal and focal adhesion disruption. EspC-producing EPEC is able to cleave fodrin, paxillin, and focal adhesion kinase (FAK), but these effects are not observed when cells are infected with an espC isogenic mutant. Recovery of these phenotypes by complementing the mutant with the espC gene but not with the espC gene mutated in the serine protease motif highlights the role of the protease activity of EspC in the cell detachment phenotype. In vitro assays using purified proteins showed that EspC, but not EspC with an S256I substitution [EspCS256I], directly cleaves these cytoskeletal and focal adhesion proteins. Kinetics of protein degradation indicated that EspC-producing EPEC first cleaves fodrin (within the 11th and 9th repetitive units at the Q1219 and D938 residues, respectively), and this event sequentially triggers paxillin degradation, FAK dephosphorylation, and FAK degradation. Thus, cytoskeletal and focal adhesion protein cleavage leads to the cell rounding and cell detachment promoted by EspC-producing EPEC." @default.
- W2024558947 created "2016-06-24" @default.
- W2024558947 creator A5019886966 @default.
- W2024558947 creator A5038969443 @default.
- W2024558947 creator A5081899651 @default.
- W2024558947 creator A5082544129 @default.
- W2024558947 creator A5084647678 @default.
- W2024558947 date "2014-06-01" @default.
- W2024558947 modified "2023-10-16" @default.
- W2024558947 title "EspC Promotes Epithelial Cell Detachment by Enteropathogenic Escherichia coli via Sequential Cleavages of a Cytoskeletal Protein and then Focal Adhesion Proteins" @default.
- W2024558947 cites W1481472473 @default.
- W2024558947 cites W1591066990 @default.
- W2024558947 cites W1913315326 @default.
- W2024558947 cites W1920045757 @default.
- W2024558947 cites W1947587196 @default.
- W2024558947 cites W1966081550 @default.
- W2024558947 cites W1966597734 @default.
- W2024558947 cites W1969527705 @default.
- W2024558947 cites W1975057095 @default.
- W2024558947 cites W1979615338 @default.
- W2024558947 cites W1981129391 @default.
- W2024558947 cites W1988771590 @default.
- W2024558947 cites W2006230005 @default.
- W2024558947 cites W2008186920 @default.
- W2024558947 cites W2009924947 @default.
- W2024558947 cites W2018077412 @default.
- W2024558947 cites W2032306900 @default.
- W2024558947 cites W2036436474 @default.
- W2024558947 cites W2050224299 @default.
- W2024558947 cites W2050499271 @default.
- W2024558947 cites W2051347791 @default.
- W2024558947 cites W2053984360 @default.
- W2024558947 cites W2057132767 @default.
- W2024558947 cites W2062734560 @default.
- W2024558947 cites W2064825875 @default.
- W2024558947 cites W2065486499 @default.
- W2024558947 cites W2067802543 @default.
- W2024558947 cites W2073376843 @default.
- W2024558947 cites W2082011034 @default.
- W2024558947 cites W2096591442 @default.
- W2024558947 cites W2099768150 @default.
- W2024558947 cites W2102627208 @default.
- W2024558947 cites W2110843117 @default.
- W2024558947 cites W2118810569 @default.
- W2024558947 cites W2125329578 @default.
- W2024558947 cites W2125618705 @default.
- W2024558947 cites W2125726407 @default.
- W2024558947 cites W2129342410 @default.
- W2024558947 cites W2130278011 @default.
- W2024558947 cites W2135428422 @default.
- W2024558947 cites W2135894069 @default.
- W2024558947 cites W2137475137 @default.
- W2024558947 cites W2154073840 @default.
- W2024558947 cites W2157116022 @default.
- W2024558947 cites W2158266769 @default.
- W2024558947 cites W2169030610 @default.
- W2024558947 cites W2171098106 @default.
- W2024558947 cites W2171120638 @default.
- W2024558947 cites W2188190921 @default.
- W2024558947 cites W4293247451 @default.
- W2024558947 doi "https://doi.org/10.1128/iai.01386-13" @default.
- W2024558947 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/4019189" @default.
- W2024558947 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24643541" @default.
- W2024558947 hasPublicationYear "2014" @default.
- W2024558947 type Work @default.
- W2024558947 sameAs 2024558947 @default.
- W2024558947 citedByCount "32" @default.
- W2024558947 countsByYear W20245589472014 @default.
- W2024558947 countsByYear W20245589472015 @default.
- W2024558947 countsByYear W20245589472016 @default.
- W2024558947 countsByYear W20245589472017 @default.
- W2024558947 countsByYear W20245589472018 @default.
- W2024558947 countsByYear W20245589472019 @default.
- W2024558947 countsByYear W20245589472020 @default.
- W2024558947 countsByYear W20245589472021 @default.
- W2024558947 countsByYear W20245589472022 @default.
- W2024558947 countsByYear W20245589472023 @default.
- W2024558947 crossrefType "journal-article" @default.
- W2024558947 hasAuthorship W2024558947A5019886966 @default.
- W2024558947 hasAuthorship W2024558947A5038969443 @default.
- W2024558947 hasAuthorship W2024558947A5081899651 @default.
- W2024558947 hasAuthorship W2024558947A5082544129 @default.
- W2024558947 hasAuthorship W2024558947A5084647678 @default.
- W2024558947 hasBestOaLocation W20245589471 @default.
- W2024558947 hasConcept C104317684 @default.
- W2024558947 hasConcept C118815799 @default.
- W2024558947 hasConcept C11960822 @default.
- W2024558947 hasConcept C142669718 @default.
- W2024558947 hasConcept C1491633281 @default.
- W2024558947 hasConcept C151166631 @default.
- W2024558947 hasConcept C2778910516 @default.
- W2024558947 hasConcept C2910478007 @default.
- W2024558947 hasConcept C547475151 @default.
- W2024558947 hasConcept C55493867 @default.
- W2024558947 hasConcept C86803240 @default.
- W2024558947 hasConcept C95444343 @default.
- W2024558947 hasConceptScore W2024558947C104317684 @default.
- W2024558947 hasConceptScore W2024558947C118815799 @default.