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• W2024573833 abstract "Abstract The incorporation of radioactivity following the application of low doses of tritiated estrogenic and non-estrogenic steroids directly to the luminal epithelia of the mouse uterus and vagina was studied. Only low percentages of the radioactivity remained in the lumina at 1 min following estradiol-17β- 3 H, estrone- 3 H, progesterone- 3 H, testosterone- 3 H and aldosterone- 3 H indicating a rapid absorption of the compounds by these tissues. The subsequent retention of radioactivity 81 min after application differed from compound to compound. Estradiol-17β showed the highest retention, 65.7%. of the injected radioactivity being retained in the uterus and 76.3% in the vagina at this time. The retention of estrone was less than that of estradiol. With all the other compounds the retention was low although in both tissues a higher retention was obtained with testosterone than with progesterone. Both these compounds were retained to a greater extent in the uterus. Estradiol showed some inhibition of the retention of radioactivity in the vagina following testosterone- 3 H. Following incubation of the same low levels of these compounds with slices of mouse uterus and vagina, there was a high incorporation of radioactivity with progesterone- 3 H, testosterone- 3 H and estradiol-17β- 3 H. There was a greater incorporation of radioactivity by the uterus than by the vagina with all three compounds, and in both tissues a greater incorporation was obtained with progesterone than with testosterone. Radioactivity from aldosterone- 3 H was not concentrated by either tissue. No interaction between estradiol, progesterone and testosterone was observed in vitro even in dose ratios of 10,000: 1. Addition of plasma or serum to the incubation medium inhibited the incorporation of radioactivity from both progesterone and estradiol although this inhibition was relatively greater with progesterone. Similarly, when plasma was added to incubations of tissues containing previously incorporated radioactivity from estradiol or progesterone, the loss of radioactivity from the tissue to the medium was greater with progesterone. With estradiol in the vagina this effect of plasma was the same for radioactivity incorporated in vivo or in vitro and it is suggested that, at least for estradiol, the incorporation observed in vitro is of the same nature as that observed in vivo ." @default.
• W2024573833 created "2016-06-24" @default.
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• W2024573833 date "1965-09-01" @default.
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• W2024573833 title "The uptake of some tritiated estrogenic and non-estrogenic steroids by the mouse uterus and vagina in vivo and in vitro" @default.
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• W2024573833 doi "https://doi.org/10.1016/0039-128x(65)90004-8" @default.
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