FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2024585162> ?p ?o ?g. }

• W2024585162 endingPage "131" @default.
• W2024585162 startingPage "121" @default.
• W2024585162 abstract "Electrophysiologically complex glial cells have been widely identified from different regions of the central nervous system and constitute a dominant glial type in juvenile mice or rats. As these cells express several types of ion channels and neurotransmitter channels that were thought to be only present in neurons, this glial cell type has attracted considerable attention. However, the actual classification of these electrophysiologically complex glial cells remains unclear. They have been speculated to be an immature astrocyte because, although these cells show positive staining for the predominantly astrocytic marker S 100beta, it has not been possible to show staining for the commonly accepted mature astrocytic marker, glial fibrillary acidic protein (GFAP). To address the question of whether these cells might express GFAP at the transcript level, we combined patch-clamp electrophysiological recording with single cell RT-PCR for GFAP mRNA in glial cells acutely isolated from 4 to 12 postnatal day rats. In fresh cell suspensions from the CA1 region, complex glial cells were found to be the dominant cell type (65% total cells). We found that the majority of these electrophysiologically complex cells (74%) were positive for GFAP mRNA. We also showed that the complex cells responded to AMPA and GABA application, and these were also GFAP mRNA positive. We also fixed and stained the preparations for GFAP without electrophysiological recording to better preserve GFAP immunoreactively. In agreement with other studies, only 1.5% of these presumed electrophysiologically complex cells, based on morphology, showed immunoreactivity for GFAP. The expression of GFAP at the transcript level indicates GFAP (-)/GFAP mRNA (+) glial cells have an astrocytic identity. As single cell RT-PCR is able to detect both GFAP (-)/GFAP mRNA (+) and GFAP (+)/GFAP mRNA (+) astrocytic subtypes, the present study also suggests it is a feasible approach for astrocytic lineage studies." @default.
• W2024585162 created "2016-06-24" @default.
• W2024585162 creator A5006355252 @default.
• W2024585162 creator A5007026581 @default.
• W2024585162 creator A5084252227 @default.
• W2024585162 date "2000-03-01" @default.
• W2024585162 modified "2023-09-27" @default.
• W2024585162 title "GFAP mRNA positive glia acutely isolated from rat hippocampus predominantly show complex current patterns" @default.
• W2024585162 cites W1599121574 @default.
• W2024585162 cites W1622863588 @default.
• W2024585162 cites W1844740521 @default.
• W2024585162 cites W1864765563 @default.
• W2024585162 cites W1967126763 @default.
• W2024585162 cites W1973476654 @default.
• W2024585162 cites W1987970152 @default.
• W2024585162 cites W1991166898 @default.
• W2024585162 cites W1993191598 @default.
• W2024585162 cites W2008577142 @default.
• W2024585162 cites W2012160176 @default.
• W2024585162 cites W2013486497 @default.
• W2024585162 cites W2019861866 @default.
• W2024585162 cites W2023402827 @default.
• W2024585162 cites W2026415168 @default.
• W2024585162 cites W2030602458 @default.
• W2024585162 cites W2037993046 @default.
• W2024585162 cites W2044139618 @default.
• W2024585162 cites W2047225757 @default.
• W2024585162 cites W2048882295 @default.
• W2024585162 cites W2058539880 @default.
• W2024585162 cites W2061210518 @default.
• W2024585162 cites W2065518413 @default.
• W2024585162 cites W2067605764 @default.
• W2024585162 cites W2068853878 @default.
• W2024585162 cites W2070169336 @default.
• W2024585162 cites W2073426624 @default.
• W2024585162 cites W2073885975 @default.
• W2024585162 cites W2083156858 @default.
• W2024585162 cites W2091570536 @default.
• W2024585162 cites W2095519131 @default.
• W2024585162 cites W2104236793 @default.
• W2024585162 cites W2126568243 @default.
• W2024585162 cites W2127303685 @default.
• W2024585162 cites W2155298124 @default.
• W2024585162 cites W2162124699 @default.
• W2024585162 cites W2169053935 @default.
• W2024585162 cites W2277121346 @default.
• W2024585162 cites W2278495047 @default.
• W2024585162 cites W2336952534 @default.
• W2024585162 doi "https://doi.org/10.1016/s0169-328x(99)00341-1" @default.
• W2024585162 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10719222" @default.
• W2024585162 hasPublicationYear "2000" @default.
• W2024585162 type Work @default.
• W2024585162 sameAs 2024585162 @default.
• W2024585162 citedByCount "37" @default.
• W2024585162 countsByYear W20245851622012 @default.
• W2024585162 countsByYear W20245851622013 @default.
• W2024585162 countsByYear W20245851622014 @default.
• W2024585162 countsByYear W20245851622015 @default.
• W2024585162 countsByYear W20245851622017 @default.
• W2024585162 countsByYear W20245851622018 @default.
• W2024585162 crossrefType "journal-article" @default.
• W2024585162 hasAuthorship W2024585162A5006355252 @default.
• W2024585162 hasAuthorship W2024585162A5007026581 @default.
• W2024585162 hasAuthorship W2024585162A5084252227 @default.
• W2024585162 hasConcept C134018914 @default.
• W2024585162 hasConcept C1491633281 @default.
• W2024585162 hasConcept C159431405 @default.
• W2024585162 hasConcept C169760540 @default.
• W2024585162 hasConcept C185263204 @default.
• W2024585162 hasConcept C189014844 @default.
• W2024585162 hasConcept C203014093 @default.
• W2024585162 hasConcept C204232928 @default.
• W2024585162 hasConcept C2777542381 @default.
• W2024585162 hasConcept C2780495277 @default.
• W2024585162 hasConcept C2780884517 @default.
• W2024585162 hasConcept C35866371 @default.
• W2024585162 hasConcept C529278444 @default.
• W2024585162 hasConcept C55493867 @default.
• W2024585162 hasConcept C86803240 @default.
• W2024585162 hasConcept C95444343 @default.
• W2024585162 hasConceptScore W2024585162C134018914 @default.
• W2024585162 hasConceptScore W2024585162C1491633281 @default.
• W2024585162 hasConceptScore W2024585162C159431405 @default.
• W2024585162 hasConceptScore W2024585162C169760540 @default.
• W2024585162 hasConceptScore W2024585162C185263204 @default.
• W2024585162 hasConceptScore W2024585162C189014844 @default.
• W2024585162 hasConceptScore W2024585162C203014093 @default.
• W2024585162 hasConceptScore W2024585162C204232928 @default.
• W2024585162 hasConceptScore W2024585162C2777542381 @default.
• W2024585162 hasConceptScore W2024585162C2780495277 @default.
• W2024585162 hasConceptScore W2024585162C2780884517 @default.
• W2024585162 hasConceptScore W2024585162C35866371 @default.
• W2024585162 hasConceptScore W2024585162C529278444 @default.
• W2024585162 hasConceptScore W2024585162C55493867 @default.
• W2024585162 hasConceptScore W2024585162C86803240 @default.
• W2024585162 hasConceptScore W2024585162C95444343 @default.
• W2024585162 hasIssue "1" @default.
• W2024585162 hasLocation W20245851621 @default.

• next