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• W2024596109 abstract "We describe several characteristics of an LM unit extracted from purified wild-type Rhodopseudomonas sphaeroides reaction centers after treatment with SDS and lauryldimethylamine N-oxide. We also studied another preparation called RC-SDS in which after a similar detergent treatment the H-chain was not separated from the LM unit. The spectral properties of both preparations were similar to those of intact reaction centers; the main differences were a blue shift of the P-865 Qy band and a narrowing of the bacteriopheophytin Qx band. Studies of absorbance changes after steady light or flash illumination showed that LM was depleted of the primary electron acceptor QI and of the transition metal (Mn), but was still able to react with quinones, with a partial restoration of the photochemistry. The primary acceptor Q10 was still partly present in RC-SDS and formed in the light a very unstable semiquinone anion. In both preparations, in the presence of added quinones, the flash-induced P-865+ decayed in the dark with multiexponential kinetics, the apparent half-times ranging between 60 ms and 100 s. This recovery was accelerated either by the absence of O2 or (in the case of LM only) by o-phenanthroline, which also decreased the amplitude of the P-865+ signal. From these experiments it was concluded that in these preparations the site of binding of the secondary quinone QII was damaged or absent; the altered properties of this LM unit were considered to result from the denaturing action of the detergents, rather than from the absence of the H polypeptide." @default.
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• W2024596109 date "1983-09-01" @default.
• W2024596109 modified "2023-09-26" @default.
• W2024596109 title "Several properties of the LM unit extracted with sodium dodecyl sulfate from Rhodopseudomonas sphaeroides purified reaction centers" @default.
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• W2024596109 doi "https://doi.org/10.1016/0005-2728(83)90093-2" @default.
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