Matches in SemOpenAlex for { <https://semopenalex.org/work/W2024609246> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W2024609246 endingPage "202" @default.
- W2024609246 startingPage "194" @default.
- W2024609246 abstract "Two experiments were conducted to study the ototoxic effects of local gentamicin (GM) administration and the subsequent hair cell (HC) regeneration process in the chinchilla cristae ampullares (CA). In the first experiment, 3 different doses of GM (0.1, 0.2 and 1.2 mg) were administered by surgical implantation of GM-soaked Gelfoam pledgets in the perilymphatic space in the otic capsule of the left superior semicircular canal. The CA was histologically processed for light-microscopic examination. In the second experiment, 6 groups of 2 chinchillas each were treated with 0.1 mg of GM. To document cell proliferation and HC regeneration, Alzet micro-osmotic pumps were implanted in each chinchilla to deliver bromodeoxyuridine (BrdU) at 125 micrograms/h for 1 week. Chinchillas were subsequently killed at 1 and 4 days and 1, 2, 4 and 8 weeks post-treatment (PT). The CA was processed for light microscopy and BrdU immunocytochemistry. In the first experiment the smallest dose produced damage restricted to HCs alone, while the medium and large doses produced severe damage in the sensory epithelium, including supporting cells and HCs. Results in the second experiment demonstrated that at 1 and 4 days PT the HCs showed extensive damage, including clumping of nuclear material. By 4 days PT the supporting cell nuclei lost their monolayer configuration. Calyceal terminals appeared empty, and vacuolized remnants of nerve calyces were evident in the basal portion. At 1 week PT complete disappearance of HCs from the sensory epithelium was evident, and there was cytoplasmic extrusion into the endolymphatic space. At 2 weeks PT there was complete HC loss, the supporting cell nuclei were scattered randomly in the crista, and the nerve fibers were retracted from the sensory epithelium. At 4 weeks PT there was evidence of sensory epithelium repair and HC regeneration. Short cells resembling type-II HCs were evident in the surface of the sensory epithelium. At 8 weeks PT the number of HCs increased in a uniform fashion on the surface of the sensory epithelium, and the supporting cell nuclei were realigned on the basal membrane. Nerve fibers with growth cones penetrated the basal membrane. Supporting cell proliferation was evident by the presence of mitotic figures and BrdU immunoreactivity in the chromatin material of dividing cells at 2 weeks PT. The labeling was more evident in newly formed cells at 4 and 8 weeks PT. These results demonstrate that in chinchillas the vestibular organs have the capacity of self-repair and the process includes HC regeneration after local administration of GM. The overall process involves changes in different cells in the sensory epithelium and neural elements, all of which show modifications with an orderly pattern." @default.
- W2024609246 created "2016-06-24" @default.
- W2024609246 creator A5011563332 @default.
- W2024609246 creator A5028259578 @default.
- W2024609246 creator A5080901166 @default.
- W2024609246 date "1995-09-01" @default.
- W2024609246 modified "2023-09-27" @default.
- W2024609246 title "Histological evidence for hair cell regeneration after ototoxic cell destruction with local application of gentamicin in the chinchilla crista ampullaris" @default.
- W2024609246 cites W1829932518 @default.
- W2024609246 cites W1909729046 @default.
- W2024609246 cites W1972017014 @default.
- W2024609246 cites W1985835614 @default.
- W2024609246 cites W1990040978 @default.
- W2024609246 cites W1992857468 @default.
- W2024609246 cites W1995048201 @default.
- W2024609246 cites W1997600733 @default.
- W2024609246 cites W2007668788 @default.
- W2024609246 cites W2019033396 @default.
- W2024609246 cites W2024964928 @default.
- W2024609246 cites W2028916500 @default.
- W2024609246 cites W2031392500 @default.
- W2024609246 cites W2041352269 @default.
- W2024609246 cites W2042791300 @default.
- W2024609246 cites W2086194510 @default.
- W2024609246 cites W2122307994 @default.
- W2024609246 cites W2319545333 @default.
- W2024609246 cites W4234957108 @default.
- W2024609246 doi "https://doi.org/10.1016/0378-5955(95)00137-7" @default.
- W2024609246 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8600126" @default.
- W2024609246 hasPublicationYear "1995" @default.
- W2024609246 type Work @default.
- W2024609246 sameAs 2024609246 @default.
- W2024609246 citedByCount "70" @default.
- W2024609246 countsByYear W20246092462012 @default.
- W2024609246 countsByYear W20246092462013 @default.
- W2024609246 countsByYear W20246092462015 @default.
- W2024609246 countsByYear W20246092462016 @default.
- W2024609246 countsByYear W20246092462017 @default.
- W2024609246 countsByYear W20246092462019 @default.
- W2024609246 countsByYear W20246092462020 @default.
- W2024609246 countsByYear W20246092462022 @default.
- W2024609246 crossrefType "journal-article" @default.
- W2024609246 hasAuthorship W2024609246A5011563332 @default.
- W2024609246 hasAuthorship W2024609246A5028259578 @default.
- W2024609246 hasAuthorship W2024609246A5080901166 @default.
- W2024609246 hasConcept C105702510 @default.
- W2024609246 hasConcept C142724271 @default.
- W2024609246 hasConcept C16685009 @default.
- W2024609246 hasConcept C171056886 @default.
- W2024609246 hasConcept C2778500370 @default.
- W2024609246 hasConcept C2778585322 @default.
- W2024609246 hasConcept C2779415040 @default.
- W2024609246 hasConcept C2780130748 @default.
- W2024609246 hasConcept C28859421 @default.
- W2024609246 hasConcept C71924100 @default.
- W2024609246 hasConcept C86803240 @default.
- W2024609246 hasConcept C95444343 @default.
- W2024609246 hasConcept C98713420 @default.
- W2024609246 hasConceptScore W2024609246C105702510 @default.
- W2024609246 hasConceptScore W2024609246C142724271 @default.
- W2024609246 hasConceptScore W2024609246C16685009 @default.
- W2024609246 hasConceptScore W2024609246C171056886 @default.
- W2024609246 hasConceptScore W2024609246C2778500370 @default.
- W2024609246 hasConceptScore W2024609246C2778585322 @default.
- W2024609246 hasConceptScore W2024609246C2779415040 @default.
- W2024609246 hasConceptScore W2024609246C2780130748 @default.
- W2024609246 hasConceptScore W2024609246C28859421 @default.
- W2024609246 hasConceptScore W2024609246C71924100 @default.
- W2024609246 hasConceptScore W2024609246C86803240 @default.
- W2024609246 hasConceptScore W2024609246C95444343 @default.
- W2024609246 hasConceptScore W2024609246C98713420 @default.
- W2024609246 hasIssue "1-2" @default.
- W2024609246 hasLocation W20246092461 @default.
- W2024609246 hasLocation W20246092462 @default.
- W2024609246 hasOpenAccess W2024609246 @default.
- W2024609246 hasPrimaryLocation W20246092461 @default.
- W2024609246 hasRelatedWork W1590553515 @default.
- W2024609246 hasRelatedWork W1969105078 @default.
- W2024609246 hasRelatedWork W1978477018 @default.
- W2024609246 hasRelatedWork W2012858508 @default.
- W2024609246 hasRelatedWork W2044613056 @default.
- W2024609246 hasRelatedWork W2065371942 @default.
- W2024609246 hasRelatedWork W2094485745 @default.
- W2024609246 hasRelatedWork W2411429672 @default.
- W2024609246 hasRelatedWork W3045360393 @default.
- W2024609246 hasRelatedWork W3145018146 @default.
- W2024609246 hasVolume "89" @default.
- W2024609246 isParatext "false" @default.
- W2024609246 isRetracted "false" @default.
- W2024609246 magId "2024609246" @default.
- W2024609246 workType "article" @default.