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- W2024609847 abstract "The hydroxylapatite-column assay of estrogen receptors, described previously [Erdos et al. Analyt. Biochem.37 244 (1970)], has been modified for the routine analyses of many samples. Sixty assays can be performed in 4h with the help of home-made filtering devices or even faster on commercial filter-racks. The method is at least as simple and rapid as its “batch” variant, it is particularly fitted for kinetic studies (the adsorption of protein and its elimination of hormone not bound to the receptor being very rapid) and allows for working with samples of large volume and low protein content (because protein is quantitatively and rapidly adsorbed even from very dilute solutions). The equilibrium association constant of the receptor can be calculated from data of experiments carried out by the hydroxylapatite-column method by the use of a correction factor, or directly, if the extract is diluted to contain less than 0.25 mg protein/ml." @default.
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- W2024609847 date "1979-03-01" @default.
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- W2024609847 title "The hydroxylapatite-column assay of estrogen receptors: The routine analyses of many samples and the calculation of the equilibrium association constant" @default.
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- W2024609847 doi "https://doi.org/10.1016/0022-4731(79)90252-8" @default.
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