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• W2024647978 abstract "The use of both proton and deuterium dipolar coupling networks to obtain efficient 13C magic-angle-spinning NMR correlation spectroscopy is introduced. This new strategy aims to improve the efficiency of 13C spin diffusion in perdeuterated proteins. The method is called double-nucleus enhanced recoupling (DONER), and it provides significantly improved transfer efficiency for carbon spin diffusion at low proton density. The Cα cross-peak intensity in the DONER experiment is ∼3 and ∼5 times stronger than those in conventional radio-frequency-assisted diffusion (RAD) and proton-driven spin diffusion (PDSD) experiments, respectively. Remarkably, the full cross-peak pattern for the aliphatic region of an extensively perdeuterated SH3 protein sample can be obtained using the DONER approach with direct 13C excitation." @default.
• W2024647978 created "2016-06-24" @default.
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• W2024647978 date "2009-11-09" @default.
• W2024647978 modified "2023-09-25" @default.
• W2024647978 title "Double-Nucleus Enhanced Recoupling for Efficient ¹³C MAS NMR Correlation Spectroscopy of Perdeuterated Proteins" @default.
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• W2024647978 doi "https://doi.org/10.1021/ja907493p" @default.
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