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- W2024650462 abstract "A novel approach for evaluation of polymerase chain reaction product was described. A biotin-labeled DNA (or RNA) probe is hybridized to the digoxigenin-labeled target PCR product. The hybrids are retained on an anti-biotin antibody-coated plate. An anti-digoxigenin Fab conjugated with alkaline phosphatase is used for generating either a fluorescence or a chromagenic substance. Both fluorogenic and chromagenic systems can be used for quantitative measurement of the PCR product/probe hybrids. The method is specific, fast, nonisotopic, sensitive, quantitative, and practical." @default.
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- W2024650462 date "1993-09-01" @default.
- W2024650462 modified "2023-10-18" @default.
- W2024650462 title "Quantitative Measurement of Nonisotopically Labeled Polymerase Chain Reaction Product" @default.
- W2024650462 doi "https://doi.org/10.1006/abio.1993.1441" @default.
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