FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2024653706> ?p ?o ?g. }

• W2024653706 endingPage "121" @default.
• W2024653706 startingPage "112" @default.
• W2024653706 abstract "Sirtuins are NAD+-dependent histone deacetylases (HDACs) that cleave off acetyl but also other acyl groups from the ϵ-amino group of lysines in histones and other substrate proteins. Five sirtuin isoforms are encoded in the genome of the parasitic pathogen Schistosoma mansoni. During its life cycle, S. mansoni undergoes drastic changes in phenotype that are associated with epigenetic modifications. Previous work showed strong effects of hSirt2 inhibitors on both worm life span and reproduction. Thus, we postulate smSirt2 as a new antiparasite target. We report both the optimization of a homogeneous fluorescence-based assay and the development of a new heterogeneous fluorescence-based assay to determine smSirt2 activity. The homogeneous assay uses a coumarin-labeled acetyl lysine derivative, and the heterogeneous version is using a biotinylated and fluorescence-labeled oligopeptide. Magnetic streptavidin-coated beads allow higher substrate loading per well than streptavidin-coated microtiter plates and make it possible to screen for inhibitors of either smSirt2 or its human isoform (hSirt2) for selectivity studies. We also present hits from a pilot screen with inhibitors showing an IC50 lower than 50 µM. Binding of the hits to their targets is rationalized by docking studies using a homology model of smSirt2." @default.
• W2024653706 created "2016-06-24" @default.
• W2024653706 creator A5000797680 @default.
• W2024653706 creator A5005295253 @default.
• W2024653706 creator A5010022616 @default.
• W2024653706 creator A5016266091 @default.
• W2024653706 creator A5055328126 @default.
• W2024653706 creator A5059430227 @default.
• W2024653706 creator A5061931567 @default.
• W2024653706 creator A5085546344 @default.
• W2024653706 creator A5087659225 @default.
• W2024653706 creator A5090497813 @default.
• W2024653706 date "2015-01-01" @default.
• W2024653706 modified "2023-10-14" @default.
• W2024653706 title "Fluorescence-Based Screening Assays for the NAD+-Dependent Histone Deacetylase smSirt2 from Schistosoma mansoni" @default.
• W2024653706 cites W1964248507 @default.
• W2024653706 cites W1964296506 @default.
• W2024653706 cites W1969967288 @default.
• W2024653706 cites W1972924731 @default.
• W2024653706 cites W1977112537 @default.
• W2024653706 cites W1981549228 @default.
• W2024653706 cites W1991001144 @default.
• W2024653706 cites W1991474023 @default.
• W2024653706 cites W2007891660 @default.
• W2024653706 cites W2008156381 @default.
• W2024653706 cites W2019071362 @default.
• W2024653706 cites W2019627057 @default.
• W2024653706 cites W2022658418 @default.
• W2024653706 cites W2039293908 @default.
• W2024653706 cites W2044033709 @default.
• W2024653706 cites W2056418498 @default.
• W2024653706 cites W2056673958 @default.
• W2024653706 cites W2071931654 @default.
• W2024653706 cites W2080626304 @default.
• W2024653706 cites W2092507534 @default.
• W2024653706 cites W2106715610 @default.
• W2024653706 cites W2110118637 @default.
• W2024653706 cites W2116350182 @default.
• W2024653706 cites W2116381732 @default.
• W2024653706 cites W2129647444 @default.
• W2024653706 cites W2139449514 @default.
• W2024653706 cites W2146739166 @default.
• W2024653706 cites W2156929149 @default.
• W2024653706 cites W2163880445 @default.
• W2024653706 cites W2169880855 @default.
• W2024653706 doi "https://doi.org/10.1177/1087057114555307" @default.
• W2024653706 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25325257" @default.
• W2024653706 hasPublicationYear "2015" @default.
• W2024653706 type Work @default.
• W2024653706 sameAs 2024653706 @default.
• W2024653706 citedByCount "17" @default.
• W2024653706 countsByYear W20246537062015 @default.
• W2024653706 countsByYear W20246537062016 @default.
• W2024653706 countsByYear W20246537062017 @default.
• W2024653706 countsByYear W20246537062018 @default.
• W2024653706 countsByYear W20246537062019 @default.
• W2024653706 countsByYear W20246537062021 @default.
• W2024653706 countsByYear W20246537062022 @default.
• W2024653706 crossrefType "journal-article" @default.
• W2024653706 hasAuthorship W2024653706A5000797680 @default.
• W2024653706 hasAuthorship W2024653706A5005295253 @default.
• W2024653706 hasAuthorship W2024653706A5010022616 @default.
• W2024653706 hasAuthorship W2024653706A5016266091 @default.
• W2024653706 hasAuthorship W2024653706A5055328126 @default.
• W2024653706 hasAuthorship W2024653706A5059430227 @default.
• W2024653706 hasAuthorship W2024653706A5061931567 @default.
• W2024653706 hasAuthorship W2024653706A5085546344 @default.
• W2024653706 hasAuthorship W2024653706A5087659225 @default.
• W2024653706 hasAuthorship W2024653706A5090497813 @default.
• W2024653706 hasBestOaLocation W20246537061 @default.
• W2024653706 hasConcept C104317684 @default.
• W2024653706 hasConcept C119157956 @default.
• W2024653706 hasConcept C153911025 @default.
• W2024653706 hasConcept C165901193 @default.
• W2024653706 hasConcept C181199279 @default.
• W2024653706 hasConcept C185592680 @default.
• W2024653706 hasConcept C199848840 @default.
• W2024653706 hasConcept C204628709 @default.
• W2024653706 hasConcept C2776016237 @default.
• W2024653706 hasConcept C2776190903 @default.
• W2024653706 hasConcept C2776225656 @default.
• W2024653706 hasConcept C2776863245 @default.
• W2024653706 hasConcept C2778204606 @default.
• W2024653706 hasConcept C2778305200 @default.
• W2024653706 hasConcept C2779085176 @default.
• W2024653706 hasConcept C2780561631 @default.
• W2024653706 hasConcept C515207424 @default.
• W2024653706 hasConcept C55493867 @default.
• W2024653706 hasConcept C64927066 @default.
• W2024653706 hasConcept C75520062 @default.
• W2024653706 hasConcept C86803240 @default.
• W2024653706 hasConcept C90856448 @default.
• W2024653706 hasConceptScore W2024653706C104317684 @default.
• W2024653706 hasConceptScore W2024653706C119157956 @default.
• W2024653706 hasConceptScore W2024653706C153911025 @default.
• W2024653706 hasConceptScore W2024653706C165901193 @default.
• W2024653706 hasConceptScore W2024653706C181199279 @default.
• W2024653706 hasConceptScore W2024653706C185592680 @default.

• next