FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2024654046> ?p ?o ?g. }

• W2024654046 endingPage "53" @default.
• W2024654046 startingPage "43" @default.
• W2024654046 abstract "SUMMARY This study evaluated the effect of three maturation media on the development of in vitro -matured and in vitro -fertilized dog oocytes. In Experiment 1 (non-comparative experiment) canine cumulus–oocyte complexes (COCs) were matured in vitro in TCM199 supplemented with estrous cow serum (10%) + gonadotropins + steroid (treatment A), TCM199 + estrous cow serum (10%) (treatment B), or TCM199 + polyvinylpyrrolidone (PVP) (4%) (treatment C). All maturation media contained a final concentration of 1 μg/ml of human somatotropin (hST). Oocytes were fertilized with fresh ejaculated sperm and development was assessed by cleavage. The objective of Experiment 2 (comparative experiment) was to compare the rates of cleavage and developmental capacity of COCs matured in vitro in same medium as in Experiment 1, and fertilized either with fresh ejaculated or with cooled extended homologous spermatozoa. In Experiments 1 and 2, oocytes fertilized with fresh semen were in vitro -matured for 48 h, while in Experiment 2 COCs fertilized with cooled semen were matured in vitro for 72 h. The results of Experiments 1 and 2 demonstrated that cleavage was not influenced by the oocyte's maturation environment. The results of Experiment 1 showed that pronucleus formation + cleavage (day 7 after IVF) was similar among treatments A, B and C ( p = 0.277). Also, in Experiment 2, pronucleus formation + cleavage (day 7 after IVF) was not different for oocytes fertilized in vitro either with fresh or cooled semen and maturated in media A ( p = 0.190), B ( p = 0.393) or C ( p = 0.687). In both experiments, the numbers of embryos that developed to the 6–8-cell stage were higher for oocytes matured in medium A and fertilized with fresh semen, when compared with numbers of oocytes matured in media B and C. Embryo development to the 6–8-cell stage of oocytes fertilized either with fresh or cooled sperm was observed in treatments A and C in Experiment 2. Cumulus cell expansion was similar among treatments in Experiment 1. In Experiment 2, cumulus cell expansion among treatments A, B and C was similar after 48 h or 72 h of IVM. In both experiments, the greatest expansion category seen was for category 2 (outer cumulus cells slightly expanded). No correlation between cumulus expansion and cleavage were observed. Polyspermy rates in oocytes matured in medium A, and fertilized with fresh sperm were not significantly different from polyspermy rates observed using media B and C, in both experiments. Our findings indicate that treatments A, B and C are similarly effective for the cleavage of dog oocytes. Furthermore, it was demonstrated that canine oocytes matured in vitro could be fertilized by homologous cooled spermatozoa and progress to cleavage." @default.
• W2024654046 created "2016-06-24" @default.
• W2024654046 creator A5051263465 @default.
• W2024654046 creator A5053162176 @default.
• W2024654046 creator A5068416026 @default.
• W2024654046 date "2007-02-01" @default.
• W2024654046 modified "2023-09-23" @default.
• W2024654046 title "Effect of maturation medium on in vitro cleavage of canine oocytes fertilized with fresh and cooled homologous semen" @default.
• W2024654046 cites W1555260014 @default.
• W2024654046 cites W1594409706 @default.
• W2024654046 cites W1963645573 @default.
• W2024654046 cites W1972905427 @default.
• W2024654046 cites W1987285745 @default.
• W2024654046 cites W1989365748 @default.
• W2024654046 cites W1990109994 @default.
• W2024654046 cites W1995361054 @default.
• W2024654046 cites W2009463087 @default.
• W2024654046 cites W2014522504 @default.
• W2024654046 cites W2015959832 @default.
• W2024654046 cites W2022181033 @default.
• W2024654046 cites W2042825195 @default.
• W2024654046 cites W2051038137 @default.
• W2024654046 cites W2067537056 @default.
• W2024654046 cites W2069158541 @default.
• W2024654046 cites W2072641768 @default.
• W2024654046 cites W2073181635 @default.
• W2024654046 cites W2074492710 @default.
• W2024654046 cites W2089142257 @default.
• W2024654046 cites W2099659034 @default.
• W2024654046 cites W2108449887 @default.
• W2024654046 cites W2110943561 @default.
• W2024654046 cites W2119349543 @default.
• W2024654046 cites W2126722856 @default.
• W2024654046 cites W2135264020 @default.
• W2024654046 cites W2135859224 @default.
• W2024654046 cites W2143297651 @default.
• W2024654046 cites W2149663546 @default.
• W2024654046 cites W2154790945 @default.
• W2024654046 cites W2160779223 @default.
• W2024654046 cites W2166713989 @default.
• W2024654046 cites W2169425331 @default.
• W2024654046 cites W2406967299 @default.
• W2024654046 cites W2618336322 @default.
• W2024654046 doi "https://doi.org/10.1017/s0967199406003960" @default.
• W2024654046 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17391545" @default.
• W2024654046 hasPublicationYear "2007" @default.
• W2024654046 type Work @default.
• W2024654046 sameAs 2024654046 @default.
• W2024654046 citedByCount "16" @default.
• W2024654046 countsByYear W20246540462012 @default.
• W2024654046 countsByYear W20246540462021 @default.
• W2024654046 crossrefType "journal-article" @default.
• W2024654046 hasAuthorship W2024654046A5051263465 @default.
• W2024654046 hasAuthorship W2024654046A5053162176 @default.
• W2024654046 hasAuthorship W2024654046A5068416026 @default.
• W2024654046 hasConcept C105702510 @default.
• W2024654046 hasConcept C110758877 @default.
• W2024654046 hasConcept C151730666 @default.
• W2024654046 hasConcept C16685009 @default.
• W2024654046 hasConcept C175156509 @default.
• W2024654046 hasConcept C185592680 @default.
• W2024654046 hasConcept C196843134 @default.
• W2024654046 hasConcept C202751555 @default.
• W2024654046 hasConcept C2775966511 @default.
• W2024654046 hasConcept C2776690073 @default.
• W2024654046 hasConcept C2776803348 @default.
• W2024654046 hasConcept C2778093475 @default.
• W2024654046 hasConcept C2778177303 @default.
• W2024654046 hasConcept C2781087480 @default.
• W2024654046 hasConcept C28748434 @default.
• W2024654046 hasConcept C2909946033 @default.
• W2024654046 hasConcept C43369102 @default.
• W2024654046 hasConcept C512716672 @default.
• W2024654046 hasConcept C55493867 @default.
• W2024654046 hasConcept C71924100 @default.
• W2024654046 hasConcept C86803240 @default.
• W2024654046 hasConcept C87073359 @default.
• W2024654046 hasConcept C88972607 @default.
• W2024654046 hasConcept C95444343 @default.
• W2024654046 hasConceptScore W2024654046C105702510 @default.
• W2024654046 hasConceptScore W2024654046C110758877 @default.
• W2024654046 hasConceptScore W2024654046C151730666 @default.
• W2024654046 hasConceptScore W2024654046C16685009 @default.
• W2024654046 hasConceptScore W2024654046C175156509 @default.
• W2024654046 hasConceptScore W2024654046C185592680 @default.
• W2024654046 hasConceptScore W2024654046C196843134 @default.
• W2024654046 hasConceptScore W2024654046C202751555 @default.
• W2024654046 hasConceptScore W2024654046C2775966511 @default.
• W2024654046 hasConceptScore W2024654046C2776690073 @default.
• W2024654046 hasConceptScore W2024654046C2776803348 @default.
• W2024654046 hasConceptScore W2024654046C2778093475 @default.
• W2024654046 hasConceptScore W2024654046C2778177303 @default.
• W2024654046 hasConceptScore W2024654046C2781087480 @default.
• W2024654046 hasConceptScore W2024654046C28748434 @default.
• W2024654046 hasConceptScore W2024654046C2909946033 @default.
• W2024654046 hasConceptScore W2024654046C43369102 @default.
• W2024654046 hasConceptScore W2024654046C512716672 @default.
• W2024654046 hasConceptScore W2024654046C55493867 @default.

• next