FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2024662451> ?p ?o ?g. }

• W2024662451 abstract "DNA cloning vectors were developed which utilize the replication origin (ori) of bacteriophage fd for their propagation. These vectors depend on the expression of viral gene 2 that was inserted into phage lambda, which in turn was integrated into the host genome. The constitutive expression of gene 2 in the host cells is sufficient for the propagation of at least 100 pfd plasmids per cell. In addition to the fd ori, the pfd vectors carry various antibiotic-resistance genes and unique restriction sites. Some of these vectors have no homologies to commonly used pBR plasmids or to lambda DNA. The nucleotide sequence of the vectors can be deduced from published sequences. Large DNA inserts can be stably propagated in pfd vectors; these are more stable than similar DNA fragments cloned in intact genomes of filamentous bacteriophage. Inclusion of phage sequences required for efficient phage packaging and infection with a helper phage resulted in formation of phage particles containing single-stranded plasmid genomes. Growth at 42 degrees C without selective pressure results in loss of pfd plasmids." @default.
• W2024662451 created "2016-06-24" @default.
• W2024662451 creator A5057195222 @default.
• W2024662451 creator A5065896962 @default.
• W2024662451 creator A5071239201 @default.
• W2024662451 creator A5077658794 @default.
• W2024662451 date "1985-01-01" @default.
• W2024662451 modified "2023-09-26" @default.
• W2024662451 title "A plasmid cloning system utilizing replication and packaging functions of the filamentous bacteriophage fd" @default.
• W2024662451 cites W1498519292 @default.
• W2024662451 cites W1601687245 @default.
• W2024662451 cites W1965811724 @default.
• W2024662451 cites W1981283118 @default.
• W2024662451 cites W1982419880 @default.
• W2024662451 cites W1995347225 @default.
• W2024662451 cites W2004977857 @default.
• W2024662451 cites W2008936546 @default.
• W2024662451 cites W2016327502 @default.
• W2024662451 cites W2023149549 @default.
• W2024662451 cites W2025605962 @default.
• W2024662451 cites W2042167090 @default.
• W2024662451 cites W2045315686 @default.
• W2024662451 cites W2050615768 @default.
• W2024662451 cites W2064988395 @default.
• W2024662451 cites W2113287359 @default.
• W2024662451 cites W2132417094 @default.
• W2024662451 cites W2141653906 @default.
• W2024662451 cites W2143301841 @default.
• W2024662451 cites W2144206181 @default.
• W2024662451 cites W2161095945 @default.
• W2024662451 cites W2164198162 @default.
• W2024662451 cites W2168422276 @default.
• W2024662451 cites W2196869921 @default.
• W2024662451 doi "https://doi.org/10.1016/0378-1119(85)90242-2" @default.
• W2024662451 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2989095" @default.
• W2024662451 hasPublicationYear "1985" @default.
• W2024662451 type Work @default.
• W2024662451 sameAs 2024662451 @default.
• W2024662451 citedByCount "49" @default.
• W2024662451 countsByYear W20246624512012 @default.
• W2024662451 crossrefType "journal-article" @default.
• W2024662451 hasAuthorship W2024662451A5057195222 @default.
• W2024662451 hasAuthorship W2024662451A5065896962 @default.
• W2024662451 hasAuthorship W2024662451A5071239201 @default.
• W2024662451 hasAuthorship W2024662451A5077658794 @default.
• W2024662451 hasConcept C10079403 @default.
• W2024662451 hasConcept C104317684 @default.
• W2024662451 hasConcept C121050878 @default.
• W2024662451 hasConcept C141231307 @default.
• W2024662451 hasConcept C150194340 @default.
• W2024662451 hasConcept C153911025 @default.
• W2024662451 hasConcept C168374021 @default.
• W2024662451 hasConcept C19924922 @default.
• W2024662451 hasConcept C199360897 @default.
• W2024662451 hasConcept C203284983 @default.
• W2024662451 hasConcept C22744801 @default.
• W2024662451 hasConcept C2776441376 @default.
• W2024662451 hasConcept C2781135666 @default.
• W2024662451 hasConcept C40767141 @default.
• W2024662451 hasConcept C41008148 @default.
• W2024662451 hasConcept C54355233 @default.
• W2024662451 hasConcept C547475151 @default.
• W2024662451 hasConcept C552990157 @default.
• W2024662451 hasConcept C58337764 @default.
• W2024662451 hasConcept C80343103 @default.
• W2024662451 hasConcept C86803240 @default.
• W2024662451 hasConcept C92087593 @default.
• W2024662451 hasConceptScore W2024662451C10079403 @default.
• W2024662451 hasConceptScore W2024662451C104317684 @default.
• W2024662451 hasConceptScore W2024662451C121050878 @default.
• W2024662451 hasConceptScore W2024662451C141231307 @default.
• W2024662451 hasConceptScore W2024662451C150194340 @default.
• W2024662451 hasConceptScore W2024662451C153911025 @default.
• W2024662451 hasConceptScore W2024662451C168374021 @default.
• W2024662451 hasConceptScore W2024662451C19924922 @default.
• W2024662451 hasConceptScore W2024662451C199360897 @default.
• W2024662451 hasConceptScore W2024662451C203284983 @default.
• W2024662451 hasConceptScore W2024662451C22744801 @default.
• W2024662451 hasConceptScore W2024662451C2776441376 @default.
• W2024662451 hasConceptScore W2024662451C2781135666 @default.
• W2024662451 hasConceptScore W2024662451C40767141 @default.
• W2024662451 hasConceptScore W2024662451C41008148 @default.
• W2024662451 hasConceptScore W2024662451C54355233 @default.
• W2024662451 hasConceptScore W2024662451C547475151 @default.
• W2024662451 hasConceptScore W2024662451C552990157 @default.
• W2024662451 hasConceptScore W2024662451C58337764 @default.
• W2024662451 hasConceptScore W2024662451C80343103 @default.
• W2024662451 hasConceptScore W2024662451C86803240 @default.
• W2024662451 hasConceptScore W2024662451C92087593 @default.
• W2024662451 hasLocation W20246624511 @default.
• W2024662451 hasLocation W20246624512 @default.
• W2024662451 hasOpenAccess W2024662451 @default.
• W2024662451 hasPrimaryLocation W20246624511 @default.
• W2024662451 hasRelatedWork W141266584 @default.
• W2024662451 hasRelatedWork W151975688 @default.
• W2024662451 hasRelatedWork W1707237046 @default.
• W2024662451 hasRelatedWork W1866533 @default.
• W2024662451 hasRelatedWork W2000908374 @default.
• W2024662451 hasRelatedWork W2013101893 @default.
• W2024662451 hasRelatedWork W2017835498 @default.

• next