FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2024693005> ?p ?o ?g. }

• W2024693005 endingPage "383" @default.
• W2024693005 startingPage "369" @default.
• W2024693005 abstract "Quantitation of proteins in finished natural rubber latex (NRL) products is essential in predicting their allergenic potential. The ASTM standard Modified Lowry method for measuring total protein content has been used for several years. Most recently, ASTM published a standard for more sensitive and more specific enzyme immunoassay for quantitation of antigenic NRL proteins. It is an ELISA inhibition assay, using rabbit anti NRL sera. Since the measurement of proteins in this method depends on recognition capacity of rabbit antibodies, the selection of an appropriate protein source for rabbit immunization is crucial for the accuracy of such test. In this study, we evaluated the composition of NRL proteins from ammoniated (AL) and nonammoniated (NAL) raw latex and from finished NRL products, and compared the effectiveness of sera from rabbits immunized with NRL proteins, to react with those extracts. Immune rabbit sera were analyzed by immunoblotting against extracts of several samples of AL, NAL, and glove proteins. In the NAL extracts, we identified 26-28 protein bands by SDS-PAGE. AL samples had between 6 and 9 bands with a great variation in the band positions among the samples. The Western blot analysis showed that anti-AL rabbit serum reacted with 4-9 protein bands in various AL extracts. The highest intensity of reaction was observed with the extract used to immunize the rabbits. Similar reaction was observed with anti-NAL serum. However, when the antisera were blotted against NAL extracts, anti-NAL serum reacted more strongly and with a larger number of proteins than anti-AL serum. In summary, anti-NAL serum recognized an equal number of proteins in AL extract as anti-AL serum. However, anti-AL serum recognized fewer protein molecules in NAL extract than anti-NAL serum. Our findings suggest that NAL extract contains more individual proteins than other extracts, and sera from rabbits immunized with this antigen have a greater capacity to react with a wide spectrum of NRL proteins. This finding may be helpful in selecting the representative reference antigen and antiserum for further efforts in NRL protein quantitation." @default.
• W2024693005 created "2016-06-24" @default.
• W2024693005 creator A5052462127 @default.
• W2024693005 creator A5068843435 @default.
• W2024693005 date "2002-08-29" @default.
• W2024693005 modified "2023-09-25" @default.
• W2024693005 title "IDENTIFICATION OF ANTIGENIC AND ALLERGENIC NATURAL RUBBER LATEX PROTEINS BY IMMUNOBLOTTING1*" @default.
• W2024693005 cites W1568951889 @default.
• W2024693005 cites W1975179058 @default.
• W2024693005 cites W1979337702 @default.
• W2024693005 cites W2003421017 @default.
• W2024693005 cites W2027335259 @default.
• W2024693005 cites W2035476000 @default.
• W2024693005 cites W2049594230 @default.
• W2024693005 cites W2067030459 @default.
• W2024693005 cites W2068570773 @default.
• W2024693005 cites W2070090453 @default.
• W2024693005 cites W2071827365 @default.
• W2024693005 cites W2076664115 @default.
• W2024693005 cites W2089753422 @default.
• W2024693005 cites W2090430118 @default.
• W2024693005 cites W2098822253 @default.
• W2024693005 cites W2108715523 @default.
• W2024693005 cites W3161889742 @default.
• W2024693005 doi "https://doi.org/10.1081/ias-120013034" @default.
• W2024693005 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/12227421" @default.
• W2024693005 hasPublicationYear "2002" @default.
• W2024693005 type Work @default.
• W2024693005 sameAs 2024693005 @default.
• W2024693005 citedByCount "4" @default.
• W2024693005 crossrefType "journal-article" @default.
• W2024693005 hasAuthorship W2024693005A5052462127 @default.
• W2024693005 hasAuthorship W2024693005A5068843435 @default.
• W2024693005 hasConcept C104317684 @default.
• W2024693005 hasConcept C147483822 @default.
• W2024693005 hasConcept C153911025 @default.
• W2024693005 hasConcept C159654299 @default.
• W2024693005 hasConcept C176933379 @default.
• W2024693005 hasConcept C178790620 @default.
• W2024693005 hasConcept C185592680 @default.
• W2024693005 hasConcept C203014093 @default.
• W2024693005 hasConcept C2776142715 @default.
• W2024693005 hasConcept C2776415932 @default.
• W2024693005 hasConcept C2780420688 @default.
• W2024693005 hasConcept C43617362 @default.
• W2024693005 hasConcept C55493867 @default.
• W2024693005 hasConcept C69130913 @default.
• W2024693005 hasConcept C86803240 @default.
• W2024693005 hasConcept C91523082 @default.
• W2024693005 hasConceptScore W2024693005C104317684 @default.
• W2024693005 hasConceptScore W2024693005C147483822 @default.
• W2024693005 hasConceptScore W2024693005C153911025 @default.
• W2024693005 hasConceptScore W2024693005C159654299 @default.
• W2024693005 hasConceptScore W2024693005C176933379 @default.
• W2024693005 hasConceptScore W2024693005C178790620 @default.
• W2024693005 hasConceptScore W2024693005C185592680 @default.
• W2024693005 hasConceptScore W2024693005C203014093 @default.
• W2024693005 hasConceptScore W2024693005C2776142715 @default.
• W2024693005 hasConceptScore W2024693005C2776415932 @default.
• W2024693005 hasConceptScore W2024693005C2780420688 @default.
• W2024693005 hasConceptScore W2024693005C43617362 @default.
• W2024693005 hasConceptScore W2024693005C55493867 @default.
• W2024693005 hasConceptScore W2024693005C69130913 @default.
• W2024693005 hasConceptScore W2024693005C86803240 @default.
• W2024693005 hasConceptScore W2024693005C91523082 @default.
• W2024693005 hasIssue "3" @default.
• W2024693005 hasLocation W20246930051 @default.
• W2024693005 hasLocation W20246930052 @default.
• W2024693005 hasOpenAccess W2024693005 @default.
• W2024693005 hasPrimaryLocation W20246930051 @default.
• W2024693005 hasRelatedWork W1589286368 @default.
• W2024693005 hasRelatedWork W1973692620 @default.
• W2024693005 hasRelatedWork W2080827293 @default.
• W2024693005 hasRelatedWork W2084188679 @default.
• W2024693005 hasRelatedWork W2146940419 @default.
• W2024693005 hasRelatedWork W2239019462 @default.
• W2024693005 hasRelatedWork W2359730144 @default.
• W2024693005 hasRelatedWork W2375480979 @default.
• W2024693005 hasRelatedWork W2409452022 @default.
• W2024693005 hasRelatedWork W4313376013 @default.
• W2024693005 hasVolume "23" @default.
• W2024693005 isParatext "false" @default.
• W2024693005 isRetracted "false" @default.
• W2024693005 magId "2024693005" @default.
• W2024693005 workType "article" @default.