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- W2024694205 abstract "The present paper describes and compares methods for the isolation of tobacco leaf nuclei. In contrast to standard procedures involving a long preincubation, a rapid cold ether treatment was found to aid in the release of nuclei in high yield. Two simple and rapid purification procedures are described which avoid the use of an ultracentrifuge. Relatively pure nuclei could be obtained in high yield in approximately 2 hr using the shorter purification procedures. The isolated nuclei were of acceptable quality as determined by (1) their appearance in the electron microscope, (2) their RNA, DNA, and protein content, and (3) their high activity in the DNA-dependent RNA polymerase reaction. Furthermore such preparations retained high RNA polymerase activity after at least 3 months at −20°C." @default.
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- W2024694205 date "1972-09-01" @default.
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- W2024694205 title "Simple rapid procedures for isolation of tobacco leaf nuclei" @default.
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- W2024694205 doi "https://doi.org/10.1016/0003-2697(72)90241-2" @default.
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