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- W2024703020 abstract "Two protocols were developed that yielded purified oxygen-evolving thylakoid membranes from the diatom Cylindrotheca fusiformis. One protocol employed sonication, while the second involved French press lysis of protoplasts formed by brief culture of cells in a cation-depleted medium. Regardless of the method of cell breakage, some damage to electron transport components occurred. For preservation of both light-dependent electron transport activity and in vivo fluorescence properties, 2 M sorbitol proved to be more effective than 1 M sorbitol, regardless of the method used for cell lysis. Thylakoids purified in 2 M sorbitol using the protoplast/French press method showed the best preservation of in vivo fluorescence emission signals and Photosystem II activity with ferricyanide was completely inhibited by DCMU. Thylakoids purified in 2 M sorbitol using sonication had higher rates of Photosystem II activity with ferricyanide, but this activity was less sensitive to DCMU. Whole-chain electron transport activity was low in all preparations. This is the first report of O2 evolution and of long-wavelength fluorescence in purified thylakoids of any chromophytic alga." @default.
- W2024703020 created "2016-06-24" @default.
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- W2024703020 date "1998-12-01" @default.
- W2024703020 modified "2023-09-30" @default.
- W2024703020 title "Oxygen-evolving diatom thylakoid membranes" @default.
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- W2024703020 doi "https://doi.org/10.1016/s0005-2728(98)00153-4" @default.
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