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• W2024705718 abstract "High-resolution small-angle X-ray scattering (SAXS), complemented by small-angle neutron scattering (SANS) and dynamic light scattering (DLS) experiments, was used to study the effect of curvature on the bilayer structure of dioleoyl-phosphatidylcholine (DOPC) and dioleoyl-phosphatidylserine (DOPS) unilamellar vesicles (ULVs). Bilayer curvature, as a result of finite vesicle size, was varied as a function of vesicle radius and determined by DLS and SANS measurements. Unilamellarity of large DOPC ULVs was achieved by the addition of small amounts (up to 4 mol %) of the charged lipid, DOPS. A comparison of SANS data over the range of 0.02 < q <0.2 Å-1 indicated no change in the overall bilayer thickness as a function of ULV diameter (620 to 1840 Å). SANS data were corroborated by high-resolution (0.06 < q <0.6 Å-1) SAXS data for the same diameter ULVs and data obtained from planar samples of aligned bilayers. Both the inner and outer leaflets of the bilayer were found to be indistinguishable. This observation agrees well with simple geometric models describing the effect of vesicle curvature. However, 1220-Å-diameter pure DOPS ULVs form asymmetric bilayers whose structure can most likely be rationalized in terms of geometrical constraints coupled with electrostatic interactions, rather than curvature alone." @default.
• W2024705718 created "2016-06-24" @default.
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• W2024705718 date "2006-12-08" @default.
• W2024705718 modified "2023-10-17" @default.
• W2024705718 title "Curvature Effect on the Structure of Phospholipid Bilayers" @default.
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• W2024705718 doi "https://doi.org/10.1021/la062455t" @default.
• W2024705718 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2720570" @default.
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