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- W2024705976 abstract "Genetically encoded bioluminescent indicators for intracellular Ca2+ are described here with CaM-M13 interaction-induced complementation of split Renilla luciferase. The Ca2+-induced interaction between CaM and M13 leads to complementation of the N- and C-terminal halves of split Renilla luciferase in living cells. This intramolecular interaction results in the spontaneous and simultaneous emission of bioluminescence split Renilla luciferase. This is how intracellular Ca2+ is illuminated with the intramolecular complementation of split Renilla luciferase. The Ca2+-dependent spontaneous and simultaneous emission of bioluminescence promises to reveal Ca2+ dynamics in living cells, and also in vivo using the present indicators." @default.
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- W2024705976 date "2008-11-01" @default.
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- W2024705976 title "Bioluminescent Indicators for Ca2+ Based on Split Renilla Luciferase Complementation in Living Cells" @default.
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- W2024705976 doi "https://doi.org/10.2116/analsci.24.1405" @default.
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