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• W2024746774 abstract "Legionella pneumophila is an intracellular pathogen that multiplies in a specialized vacuole within host cells. Biogenesis of this vacuole requires the Dot/Icm type IV protein translocation system. By using a Cre/loxP-based protein translocation assay, we found that proteins translocated by the Dot/Icm complex across the host phagosomal membrane can also be transferred from one bacterial cell to another. The flexibility of this system allowed the identification of several families of proteins translocated by the Dot/Icm complex. When analyzed by immunofluorescence microscopy, a protein identified by this procedure, SidC, was shown to translocate across the phagosomal membranes to the cytoplasmic face of the L. pneumophila phagosome. The identification of large numbers of these substrates, and the fact that the absence of any one substrate rarely results in strong defects in intracellular growth, indicate that there is significant functional redundancy among the Dot/Icm translocation targets." @default.
• W2024746774 created "2016-06-24" @default.
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• W2024746774 date "2004-01-08" @default.
• W2024746774 modified "2023-10-06" @default.
• W2024746774 title "Multiple substrates of the <i>Legionella pneumophila</i> Dot/Icm system identified by interbacterial protein transfer" @default.
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• W2024746774 doi "https://doi.org/10.1073/pnas.0304916101" @default.
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