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- W2024748253 abstract "A sensitive capillary gas chromatographic method was developed for the simultaneous determination of pentachlorophenol and its major metabolite, tetrachlorohydroquinone, in plasma, urine and feces. The method involved a simple one-step liquid-liquid extraction with diethyl ether and electron-capture detection gas chromatography on a fused-silica capillary column coated with 50% methylsilicone-50% trifluoropropylsilicone. The detection limit of both compounds was 50 ng/ml in plasma (from an initial volume of 0.1 ml), 100 ng/ml in urine and 100 ng/g in feces. Optimal conditions for both chemical and enzymatic hydrolysis were defined to measure conjugates of both pentachlorophenol and tetrachlorohydroquinone in urine. Tetrachlorohydroquinone was found to be unstable in plasma and urine; means to prevent its degradation during sample collection and storage by addition of ascorbic acid and ethylenediaminetetra-acetic acid are presented. This chromatographic method was shown to be precise, accurate and specific. It was successfully applied to toxicokinetic studies in rat." @default.
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- W2024748253 date "1990-01-01" @default.
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- W2024748253 title "Simultaneous assay of pentachlorophenol and its metabolite, tetrachlorohydroquinone, by gas chromatography without derivatization" @default.
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- W2024748253 doi "https://doi.org/10.1016/s0378-4347(00)82191-x" @default.
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