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- W2024750897 startingPage "1935" @default.
- W2024750897 abstract "The production of deoxyribonucleoside triphosphates (dNTPs) from deoxyribonucleoside monophosphates (dNMPs) was investigated using dried baker’s yeast in the presence of a high concentration of inorganic phosphate. The amount of dATP formed by 150mg/ml of yeast cells was 127 mm, with a 84% yield of the substrate, dAMP, after 18 hr incubation at 28°C with 150 mm dAMP, 800 mm glucose, 800 mm potassium phosphate buffer (pH 7.0) and 10 mm MgS04. The amount of dGTP or dCTP formed was in the range of 26~34mm, from 100 mm of either dGMP or dCMP, respectively, after 8 hr incubation, but dTTP was not formed during the incubation period.In a reaction mixture containing a dNMP-mixture, which was prepared through the hydrolysis of salmon testes DNA or herring sperm DNA (100 mg/ml) with Nuclease Pls 32 ~ 49 mm dNTP was obtained." @default.
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- W2024750897 date "1989-01-01" @default.
- W2024750897 modified "2023-10-03" @default.
- W2024750897 title "Production of deoxyribonucleoside triphosphates through coupled fermentation with energy transfer." @default.
- W2024750897 doi "https://doi.org/10.1271/bbb1961.53.1935" @default.
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