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- W2024760194 abstract "The tissue-specific accumulation of small bristles (Dm nxf1) transcripts at different developmental stages of Drosophila melanogaster was analyzed by Northern blots and RT PCR. We identified four distinct transcripts: ubiquitous (3.5kb); ovary and early embryo specific (3.3kb); testis specific (1.9kb and 2.8kb) and nervous system specific (5.1kb). The pattern of Dm nxf1 gene expression in ovaries and early embryos (0-2h) is similar: the sizes of transcripts range from 3.0 to 3.5kb. We propose that this size variability may reflect the different extent of cytoplasmic polyadenylation. In testes, the 2.8-kb transcript originates from alternative termination of transcription and the 1.9-kb transcript is supposed to originate from an alternative transcription start. During ontogenesis, the 5.1-kb transcript can be clearly detected in 10- to 18-h-old embryos, most prominently in the nervous ganglia of larvae, and it represents a major species in imago head extracts. We found that the 5.1-kb transcript, similarly to the nxf1 heavy transcripts in Homo sapiens and Mus musculus, results from the retention of intron 5-6 that corresponds to the intron 10-11 in Hs nxf1 and Mm nxf1 genes." @default.
- W2024760194 created "2016-06-24" @default.
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- W2024760194 date "2010-06-01" @default.
- W2024760194 modified "2023-10-07" @default.
- W2024760194 title "Alternative transcripts expressed by small bristles, the Drosophila melanogaster nxf1 gene" @default.
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- W2024760194 doi "https://doi.org/10.1016/j.gene.2010.02.013" @default.
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