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- W2024771285 abstract "This study describes the development of a real-time PCR protocol for rapid detection of the most common blaVIM (blaVIM-1, blaVIM-2, blaVIM-3, blaVIM-4, blaVIM-5, blaVIM-6, blaVIM-10, blaVIM-11, blaVIM-12) and blaIMP (blaIMP-1, blaIMP-2, blaIMP-6, blaIMP-8, blaIMP-10, blaIMP-15, blaIMP-19, blaIMP-20) genes in a single reaction. The genes were specifically detected and clearly differentiated into four groups, i.e., (i) blaVIM-1-like (blaVIM-1, blaVIM-4, blaVIM-5, blaVIM-12); (ii) blaVIM-2-like (blaVIM-2, blaVIM-3, blaVIM-6, blaVIM-10, blaVIM-11); (iii) blaIMP-1-like (blaIMP-1, blaIMP-6, blaIMP-10); and (iv) blaIMP-2-like (blaIMP-2, blaIMP-8, blaIMP-15, blaIMP-19, blaIMP-20), by melting curve analysis of the real-time PCR products. The protocol was used to screen positive blaVIM-1, blaVIM-2 and blaIMP-1 control strains, 70 Gram-negative isolates resistant to carbapenems, and 30 Gram-negative isolates susceptible to carbapenems (negative controls)." @default.
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- W2024771285 date "2007-12-01" @default.
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- W2024771285 title "Specific detection of blaVIM and blaIMP metallo-β-lactamase genes in a single real-time PCR" @default.
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- W2024771285 doi "https://doi.org/10.1111/j.1469-0691.2007.01832.x" @default.
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