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• W2024791560 startingPage "711" @default.
• W2024791560 abstract "Virus-transformed pre-B cells undergo ordered immunoglobulin (Ig) gene rearrangements during culture. We devised a series of highly sensitive polymerase chain reaction assays for Ig gene rearrangement and unrearranged Ig gene segment transcription to study both the possible relationship between these processes in cultured pre-B cells and the role played by heavy (H) chain (mu) protein in regulating gene rearrangement. Our analysis of pre-B cell cultures representing various stages of maturity revealed that transcription of each germline Ig locus precedes or is coincident with its rearrangement. Cell lines containing one functional rearranged H chain allele, however, continue to transcribe and to rearrange the allelic, unrearranged H chain locus. These cell lines appear to initiate but not terminate rearrangement events and therefore provide information about the requirements for activating rearrangement but not about allelic exclusion mechanisms." @default.
• W2024791560 created "2016-06-24" @default.
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• W2024791560 date "1991-03-01" @default.
• W2024791560 modified "2023-09-26" @default.
• W2024791560 title "Virus-transformed pre-B cells show ordered activation but not inactivation of immunoglobulin gene rearrangement and transcription." @default.
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• W2024791560 doi "https://doi.org/10.1084/jem.173.3.711" @default.
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