FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2024794798> ?p ?o ?g. }

• W2024794798 endingPage "290" @default.
• W2024794798 startingPage "283" @default.
• W2024794798 abstract "Using in situ hybridisation histochemistry in combination with patch-clamp recordings and specific pharmacological tools, the molecular nature of the channels underlying Ca2+-dependent K+ currents was determined in dorsal vagal neurones (DVNs) of rat brainstem slices. In situ hybridisation analysis at cellular resolution revealed the presence of 'big'-conductance Ca2+- and voltage-activated K+ (BK) channel alpha-subunit mRNA, and of only one 'small'-conductance Ca2+-activated K+ (SK) channel subunit transcript, SK3, at very high levels in DVNs. By contrast, SK1 and SK2 mRNAs were below the threshold limit of detection. The SK channel-mediated after-hyperpolarising current (IAHP) was blocked by apamin with a half-maximal inhibitory concentration of approximately 2.2 nM. This is consistent with homomultimeric SK3 channels mediating IAHP in DVNs. IAHP was also blocked by scyllatoxin (20-30 nM) and curare (100-200 microM). Application of apamin (100 nM) or scyllatoxin (20 nM) invariably caused a substantial increase to 146.1 +/- 10.4 and 181.8 +/- 12.9 % of control, respectively, in the spontaneous firing rate of DVNs. Action potential duration was not affected by these SK channel blockers. The selective BK channel blocker iberiotoxin (50 nM) increased action potential duration by 22.5 +/- 7.3 %, as did low concentrations of tetraethylammonium (0.5 mM; 99.3 +/- 16.4 %) and the Ca2+ channel blocker Cd2+ (100 microM; 49.5 +/- 20.9 %). BK channel blockade did not significantly affect the firing rate of DVNs. These results allow us to establish a tight correlation between the properties of cloned and native BK and SK channels, and to achieve an understanding, at the molecular level, of their role in regulating the spontaneous firing frequency and in shaping single action potentials of central neurones." @default.
• W2024794798 created "2016-06-24" @default.
• W2024794798 creator A5012985523 @default.
• W2024794798 creator A5014252679 @default.
• W2024794798 creator A5029600467 @default.
• W2024794798 creator A5053372363 @default.
• W2024794798 creator A5084176336 @default.
• W2024794798 date "2000-09-01" @default.
• W2024794798 modified "2023-10-15" @default.
• W2024794798 title "Molecular determinants of Ca²⁺‐dependent K⁺ channel function in rat dorsal vagal neurones" @default.
• W2024794798 cites W149934552 @default.
• W2024794798 cites W1795948620 @default.
• W2024794798 cites W181964475 @default.
• W2024794798 cites W1827142872 @default.
• W2024794798 cites W1876408798 @default.
• W2024794798 cites W1979287010 @default.
• W2024794798 cites W1987369275 @default.
• W2024794798 cites W1989712467 @default.
• W2024794798 cites W1990640328 @default.
• W2024794798 cites W1991673195 @default.
• W2024794798 cites W1994688027 @default.
• W2024794798 cites W1997867282 @default.
• W2024794798 cites W2010141254 @default.
• W2024794798 cites W2020626643 @default.
• W2024794798 cites W2021958245 @default.
• W2024794798 cites W2033183892 @default.
• W2024794798 cites W2035845442 @default.
• W2024794798 cites W2042670053 @default.
• W2024794798 cites W2051040059 @default.
• W2024794798 cites W2056552455 @default.
• W2024794798 cites W2073049800 @default.
• W2024794798 cites W2085534162 @default.
• W2024794798 cites W2119397841 @default.
• W2024794798 cites W2165892338 @default.
• W2024794798 cites W2171584675 @default.
• W2024794798 cites W2181918135 @default.
• W2024794798 cites W2336137754 @default.
• W2024794798 doi "https://doi.org/10.1111/j.1469-7793.2000.t01-1-00283.x" @default.
• W2024794798 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2270078" @default.
• W2024794798 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/10970429" @default.
• W2024794798 hasPublicationYear "2000" @default.
• W2024794798 type Work @default.
• W2024794798 sameAs 2024794798 @default.
• W2024794798 citedByCount "86" @default.
• W2024794798 countsByYear W20247947982012 @default.
• W2024794798 countsByYear W20247947982013 @default.
• W2024794798 countsByYear W20247947982014 @default.
• W2024794798 countsByYear W20247947982016 @default.
• W2024794798 countsByYear W20247947982017 @default.
• W2024794798 countsByYear W20247947982021 @default.
• W2024794798 countsByYear W20247947982022 @default.
• W2024794798 crossrefType "journal-article" @default.
• W2024794798 hasAuthorship W2024794798A5012985523 @default.
• W2024794798 hasAuthorship W2024794798A5014252679 @default.
• W2024794798 hasAuthorship W2024794798A5029600467 @default.
• W2024794798 hasAuthorship W2024794798A5053372363 @default.
• W2024794798 hasAuthorship W2024794798A5084176336 @default.
• W2024794798 hasBestOaLocation W20247947981 @default.
• W2024794798 hasConcept C121332964 @default.
• W2024794798 hasConcept C121932024 @default.
• W2024794798 hasConcept C12554922 @default.
• W2024794798 hasConcept C126322002 @default.
• W2024794798 hasConcept C134018914 @default.
• W2024794798 hasConcept C147944092 @default.
• W2024794798 hasConcept C149786707 @default.
• W2024794798 hasConcept C153461711 @default.
• W2024794798 hasConcept C170493617 @default.
• W2024794798 hasConcept C17077164 @default.
• W2024794798 hasConcept C178790620 @default.
• W2024794798 hasConcept C181911157 @default.
• W2024794798 hasConcept C185263204 @default.
• W2024794798 hasConcept C185592680 @default.
• W2024794798 hasConcept C26873012 @default.
• W2024794798 hasConcept C2776919887 @default.
• W2024794798 hasConcept C2778457506 @default.
• W2024794798 hasConcept C2779411790 @default.
• W2024794798 hasConcept C2780419564 @default.
• W2024794798 hasConcept C2781295685 @default.
• W2024794798 hasConcept C50254741 @default.
• W2024794798 hasConcept C517785266 @default.
• W2024794798 hasConcept C519063684 @default.
• W2024794798 hasConcept C55493867 @default.
• W2024794798 hasConcept C71924100 @default.
• W2024794798 hasConcept C83743174 @default.
• W2024794798 hasConcept C86803240 @default.
• W2024794798 hasConceptScore W2024794798C121332964 @default.
• W2024794798 hasConceptScore W2024794798C121932024 @default.
• W2024794798 hasConceptScore W2024794798C12554922 @default.
• W2024794798 hasConceptScore W2024794798C126322002 @default.
• W2024794798 hasConceptScore W2024794798C134018914 @default.
• W2024794798 hasConceptScore W2024794798C147944092 @default.
• W2024794798 hasConceptScore W2024794798C149786707 @default.
• W2024794798 hasConceptScore W2024794798C153461711 @default.
• W2024794798 hasConceptScore W2024794798C170493617 @default.
• W2024794798 hasConceptScore W2024794798C17077164 @default.
• W2024794798 hasConceptScore W2024794798C178790620 @default.
• W2024794798 hasConceptScore W2024794798C181911157 @default.
• W2024794798 hasConceptScore W2024794798C185263204 @default.

• next