Matches in SemOpenAlex for { <https://semopenalex.org/work/W2024795737> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W2024795737 endingPage "449" @default.
- W2024795737 startingPage "432" @default.
- W2024795737 abstract "As the initial phase of a study of mammalian catabolism of the peptide hormone gastrin, we demonstrated in rat tissues the presence of an enzymatic activity catalyzing deamidation of the phenylalanine amide of the biologically active C-terminal tetrapeptide amide fragment of gastrin (Try-Met-Asp-Phe-NH2) to produce the biologically inactive tetrapeptide free acid. Because an interfering aminopeptidase that acts on the C-terminal tetrapeptide amide was widespread in rat tissues, N-acylated BOC-tetrapeptide amide was used as the substrate for studying the amidase activity. Identification of BOC-tetrapeptide free acid as a product of the amidase reaction was achieved by thin-layer chromatography and by demonstrating that the reaction product was a substrate for carboxypeptidase A, which catalyzes liberation of phenylalanine from BOC-tetrapeptide free acid but not from BOC-tetrapeptide amide. A quantitative assay was developed to measure the generation of BOC-tetrapeptide free acid; this product and ammonia were shown to be produced in equivalent amounts during the reaction. The enzyme activity was concentrated in the microsomal cell fraction. The pH optimum for the reaction was found to be approximately 6.7. Liver was found to be a rich source of the amidase activity in the rat and this activity was also demonstrated to be present in small intestinal mucosa and several other tissues of the rat and of other mammalian species. Another enzymatic activity, most abundant in rat kidney, was found to catalyze hydrolysis of the peptide bond between methionine and aspartic acid of BOC-tetrapeptide amide. The amidase activity and the peptidase activity identified in the kidney may contribute to the inactivation of gastrin or of its biologically active degradation products in vivo." @default.
- W2024795737 created "2016-06-24" @default.
- W2024795737 creator A5006195166 @default.
- W2024795737 creator A5091680206 @default.
- W2024795737 date "1973-12-01" @default.
- W2024795737 modified "2023-09-23" @default.
- W2024795737 title "Enzymatic deamidation of the C-terminal tetrapeptide amide of gastrin by mammalian tissues" @default.
- W2024795737 cites W1428747333 @default.
- W2024795737 cites W1570612113 @default.
- W2024795737 cites W1573573401 @default.
- W2024795737 cites W1775749144 @default.
- W2024795737 cites W1822950385 @default.
- W2024795737 cites W1968886433 @default.
- W2024795737 cites W1988441601 @default.
- W2024795737 cites W1992666239 @default.
- W2024795737 cites W1995781974 @default.
- W2024795737 cites W1997166387 @default.
- W2024795737 cites W1997305818 @default.
- W2024795737 cites W2008065811 @default.
- W2024795737 cites W2009442688 @default.
- W2024795737 cites W2029050852 @default.
- W2024795737 cites W2049559574 @default.
- W2024795737 cites W2058170527 @default.
- W2024795737 cites W2059813394 @default.
- W2024795737 cites W2061354172 @default.
- W2024795737 cites W2080198272 @default.
- W2024795737 cites W2087556786 @default.
- W2024795737 cites W2124421473 @default.
- W2024795737 cites W2133596493 @default.
- W2024795737 cites W2170974856 @default.
- W2024795737 cites W2339343999 @default.
- W2024795737 cites W2405020921 @default.
- W2024795737 cites W2413379117 @default.
- W2024795737 cites W2464890863 @default.
- W2024795737 cites W2608470872 @default.
- W2024795737 cites W4301055353 @default.
- W2024795737 doi "https://doi.org/10.1016/0006-2944(73)90048-3" @default.
- W2024795737 hasPublicationYear "1973" @default.
- W2024795737 type Work @default.
- W2024795737 sameAs 2024795737 @default.
- W2024795737 citedByCount "15" @default.
- W2024795737 countsByYear W20247957372018 @default.
- W2024795737 countsByYear W20247957372020 @default.
- W2024795737 crossrefType "journal-article" @default.
- W2024795737 hasAuthorship W2024795737A5006195166 @default.
- W2024795737 hasAuthorship W2024795737A5091680206 @default.
- W2024795737 hasConcept C181199279 @default.
- W2024795737 hasConcept C185592680 @default.
- W2024795737 hasConcept C2776986829 @default.
- W2024795737 hasConcept C2777927761 @default.
- W2024795737 hasConcept C2778439535 @default.
- W2024795737 hasConcept C2779281246 @default.
- W2024795737 hasConcept C2779564779 @default.
- W2024795737 hasConcept C2780784506 @default.
- W2024795737 hasConcept C49039625 @default.
- W2024795737 hasConcept C515207424 @default.
- W2024795737 hasConcept C55493867 @default.
- W2024795737 hasConcept C60002323 @default.
- W2024795737 hasConcept C65588867 @default.
- W2024795737 hasConcept C71240020 @default.
- W2024795737 hasConceptScore W2024795737C181199279 @default.
- W2024795737 hasConceptScore W2024795737C185592680 @default.
- W2024795737 hasConceptScore W2024795737C2776986829 @default.
- W2024795737 hasConceptScore W2024795737C2777927761 @default.
- W2024795737 hasConceptScore W2024795737C2778439535 @default.
- W2024795737 hasConceptScore W2024795737C2779281246 @default.
- W2024795737 hasConceptScore W2024795737C2779564779 @default.
- W2024795737 hasConceptScore W2024795737C2780784506 @default.
- W2024795737 hasConceptScore W2024795737C49039625 @default.
- W2024795737 hasConceptScore W2024795737C515207424 @default.
- W2024795737 hasConceptScore W2024795737C55493867 @default.
- W2024795737 hasConceptScore W2024795737C60002323 @default.
- W2024795737 hasConceptScore W2024795737C65588867 @default.
- W2024795737 hasConceptScore W2024795737C71240020 @default.
- W2024795737 hasIssue "3" @default.
- W2024795737 hasLocation W20247957371 @default.
- W2024795737 hasOpenAccess W2024795737 @default.
- W2024795737 hasPrimaryLocation W20247957371 @default.
- W2024795737 hasRelatedWork W1566614166 @default.
- W2024795737 hasRelatedWork W1644178486 @default.
- W2024795737 hasRelatedWork W2024795737 @default.
- W2024795737 hasRelatedWork W2045394784 @default.
- W2024795737 hasRelatedWork W2096253353 @default.
- W2024795737 hasRelatedWork W2107792670 @default.
- W2024795737 hasRelatedWork W2161131276 @default.
- W2024795737 hasRelatedWork W2325467169 @default.
- W2024795737 hasRelatedWork W2402941385 @default.
- W2024795737 hasRelatedWork W3164186914 @default.
- W2024795737 hasVolume "8" @default.
- W2024795737 isParatext "false" @default.
- W2024795737 isRetracted "false" @default.
- W2024795737 magId "2024795737" @default.
- W2024795737 workType "article" @default.