FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2024796659> ?p ?o ?g. }

• W2024796659 endingPage "173" @default.
• W2024796659 startingPage "167" @default.
• W2024796659 abstract "Human hepatitis E virus (HEV) causes acute hepatitis in humans, predominantly by contamination of food and water. HEV, in particular genotype III, is currently considered to be an emerging pathogen in industrialized countries. Because of the low infectious dose, an efficient and rapid virus concentration method is required to detect low amounts of HEV in food and water samples for routine control. Because of the absence of a reliable cell culture method for the main enteric viruses most involved in the outbreaks, reverse transcription quantitative real time PCR (RT-qPCR) is now widely used for the detection of RNA viruses in food and water samples. One of the general requirements for viral diagnosis concerns the use of a process control to monitor the efficiency of the concentration of viral particles, the extraction of nucleic acid and the presence of the potential inhibitors of the RT-qPCR reaction. The aim of this study was to provide a rapid and sensitive method for detecting HEV in water. The method is based on viral concentration by filtration on membrane filters and direct lysis of adsorbed viruses from filters before RNA extraction and RT-qPCR amplification. We developed a one-step duplex RT-qPCR for detecting HEV and the murine norovirus (MNV-1) was used as a process control. The data show that MNV-1 offers a very reliable and simple way of monitoring false-negative results and is a valuable tool in the routine diagnostic laboratory. The limit of detection (LOD) was in the range of 700 to 3500 HEV genome copies/0.5 L bottled water and 3500 HEV genome copies/0.5 L tap water." @default.
• W2024796659 created "2016-06-24" @default.
• W2024796659 creator A5014901567 @default.
• W2024796659 creator A5019291125 @default.
• W2024796659 creator A5053965184 @default.
• W2024796659 creator A5055038744 @default.
• W2024796659 date "2012-07-01" @default.
• W2024796659 modified "2023-10-18" @default.
• W2024796659 title "Duplex RT-qPCR for the detection of hepatitis E virus in water, using a process control" @default.
• W2024796659 cites W1983088970 @default.
• W2024796659 cites W1987569551 @default.
• W2024796659 cites W1993611768 @default.
• W2024796659 cites W2005808145 @default.
• W2024796659 cites W2013083219 @default.
• W2024796659 cites W2017876935 @default.
• W2024796659 cites W2036620685 @default.
• W2024796659 cites W2040066072 @default.
• W2024796659 cites W2040499564 @default.
• W2024796659 cites W2043504537 @default.
• W2024796659 cites W2044784750 @default.
• W2024796659 cites W2055286540 @default.
• W2024796659 cites W2062074799 @default.
• W2024796659 cites W2063106424 @default.
• W2024796659 cites W2063901368 @default.
• W2024796659 cites W2066149793 @default.
• W2024796659 cites W2073760272 @default.
• W2024796659 cites W2074713056 @default.
• W2024796659 cites W2077254344 @default.
• W2024796659 cites W2077505423 @default.
• W2024796659 cites W2078850138 @default.
• W2024796659 cites W2084132188 @default.
• W2024796659 cites W2088677842 @default.
• W2024796659 cites W2089611357 @default.
• W2024796659 cites W2089743437 @default.
• W2024796659 cites W2100009633 @default.
• W2024796659 cites W2109374953 @default.
• W2024796659 cites W2121968404 @default.
• W2024796659 cites W2127143045 @default.
• W2024796659 cites W2157894507 @default.
• W2024796659 cites W2158026372 @default.
• W2024796659 cites W2158848046 @default.
• W2024796659 cites W2163419435 @default.
• W2024796659 cites W2171089351 @default.
• W2024796659 doi "https://doi.org/10.1016/j.ijfoodmicro.2012.05.001" @default.
• W2024796659 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/22633799" @default.
• W2024796659 hasPublicationYear "2012" @default.
• W2024796659 type Work @default.
• W2024796659 sameAs 2024796659 @default.
• W2024796659 citedByCount "32" @default.
• W2024796659 countsByYear W20247966592013 @default.
• W2024796659 countsByYear W20247966592014 @default.
• W2024796659 countsByYear W20247966592015 @default.
• W2024796659 countsByYear W20247966592016 @default.
• W2024796659 countsByYear W20247966592017 @default.
• W2024796659 countsByYear W20247966592019 @default.
• W2024796659 countsByYear W20247966592020 @default.
• W2024796659 countsByYear W20247966592021 @default.
• W2024796659 countsByYear W20247966592022 @default.
• W2024796659 countsByYear W20247966592023 @default.
• W2024796659 crossrefType "journal-article" @default.
• W2024796659 hasAuthorship W2024796659A5014901567 @default.
• W2024796659 hasAuthorship W2024796659A5019291125 @default.
• W2024796659 hasAuthorship W2024796659A5053965184 @default.
• W2024796659 hasAuthorship W2024796659A5055038744 @default.
• W2024796659 hasConcept C104317684 @default.
• W2024796659 hasConcept C105580179 @default.
• W2024796659 hasConcept C112190416 @default.
• W2024796659 hasConcept C119128265 @default.
• W2024796659 hasConcept C135763542 @default.
• W2024796659 hasConcept C159047783 @default.
• W2024796659 hasConcept C185592680 @default.
• W2024796659 hasConcept C24107716 @default.
• W2024796659 hasConcept C2522874641 @default.
• W2024796659 hasConcept C2775940340 @default.
• W2024796659 hasConcept C2778602366 @default.
• W2024796659 hasConcept C2778750513 @default.
• W2024796659 hasConcept C2780777367 @default.
• W2024796659 hasConcept C2994092193 @default.
• W2024796659 hasConcept C43617362 @default.
• W2024796659 hasConcept C4692481 @default.
• W2024796659 hasConcept C48023723 @default.
• W2024796659 hasConcept C55493867 @default.
• W2024796659 hasConcept C67705224 @default.
• W2024796659 hasConcept C86803240 @default.
• W2024796659 hasConcept C89423630 @default.
• W2024796659 hasConceptScore W2024796659C104317684 @default.
• W2024796659 hasConceptScore W2024796659C105580179 @default.
• W2024796659 hasConceptScore W2024796659C112190416 @default.
• W2024796659 hasConceptScore W2024796659C119128265 @default.
• W2024796659 hasConceptScore W2024796659C135763542 @default.
• W2024796659 hasConceptScore W2024796659C159047783 @default.
• W2024796659 hasConceptScore W2024796659C185592680 @default.
• W2024796659 hasConceptScore W2024796659C24107716 @default.
• W2024796659 hasConceptScore W2024796659C2522874641 @default.
• W2024796659 hasConceptScore W2024796659C2775940340 @default.
• W2024796659 hasConceptScore W2024796659C2778602366 @default.
• W2024796659 hasConceptScore W2024796659C2778750513 @default.
• W2024796659 hasConceptScore W2024796659C2780777367 @default.

• next