Matches in SemOpenAlex for { <https://semopenalex.org/work/W2024797977> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W2024797977 endingPage "420" @default.
- W2024797977 startingPage "418" @default.
- W2024797977 abstract "The napB gene of the pathogenic bacterium Haemophilus influenzae encodes a dihaem cytochrome c, the small subunit of a heterodimeric periplasmic nitrate reductase (Nap). Recombinant NapB was overproduced in Escherichia coli, purified to near-homogeneity and crystallized using the hanging-drop method. Thin quadrilateral plates were grown under various conditions but proved to be unsuitable for X-ray analysis. However, a single crystal was grown using 1.75 M ammonium sulfate in 0.1 M sodium acetate pH 5.5, from which a native data set could be collected to 1.8 A resolution using synchrotron radiation. Using the same conditions, further crystals were obtained by microseeding. The space group was determined to be P42(1)2, with unit-cell parameters a = 77.55, b = 77.55, c = 28.64 A and an unusually low solvent content of 16.5%, assuming there to be one molecule of NapB in the asymmetric unit. Analysis of the dissolved crystals indicated that partial proteolysis of the protein had occurred. Taking the molecular mass of the crystallized form ( approximately 8500 Da) into account, the solvent content was estimated to be 53%, with a V(M) value of 2.64 A(3) Da(-1)." @default.
- W2024797977 created "2016-06-24" @default.
- W2024797977 creator A5004792265 @default.
- W2024797977 creator A5027333163 @default.
- W2024797977 creator A5081598452 @default.
- W2024797977 date "2001-03-01" @default.
- W2024797977 modified "2023-09-25" @default.
- W2024797977 title "Crystallization and preliminary X-ray analysis of the recombinant dihaem cytochrome<i>c</i>(NapB) from<i>Haemophilus influenzae</i>" @default.
- W2024797977 cites W128603962 @default.
- W2024797977 cites W1531123195 @default.
- W2024797977 cites W1889431171 @default.
- W2024797977 cites W1971403296 @default.
- W2024797977 cites W1993667934 @default.
- W2024797977 cites W2006049252 @default.
- W2024797977 cites W2006990625 @default.
- W2024797977 cites W2014694459 @default.
- W2024797977 cites W2018755204 @default.
- W2024797977 cites W2030237066 @default.
- W2024797977 cites W2062521521 @default.
- W2024797977 cites W2067511893 @default.
- W2024797977 cites W2105880282 @default.
- W2024797977 cites W2139697583 @default.
- W2024797977 cites W2145262563 @default.
- W2024797977 cites W2164426273 @default.
- W2024797977 cites W254792685 @default.
- W2024797977 doi "https://doi.org/10.1107/s0907444900018011" @default.
- W2024797977 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11223519" @default.
- W2024797977 hasPublicationYear "2001" @default.
- W2024797977 type Work @default.
- W2024797977 sameAs 2024797977 @default.
- W2024797977 citedByCount "2" @default.
- W2024797977 countsByYear W20247979772015 @default.
- W2024797977 crossrefType "journal-article" @default.
- W2024797977 hasAuthorship W2024797977A5004792265 @default.
- W2024797977 hasAuthorship W2024797977A5027333163 @default.
- W2024797977 hasAuthorship W2024797977A5081598452 @default.
- W2024797977 hasConcept C104292427 @default.
- W2024797977 hasConcept C104317684 @default.
- W2024797977 hasConcept C113196181 @default.
- W2024797977 hasConcept C13965031 @default.
- W2024797977 hasConcept C153911025 @default.
- W2024797977 hasConcept C178790620 @default.
- W2024797977 hasConcept C181199279 @default.
- W2024797977 hasConcept C185592680 @default.
- W2024797977 hasConcept C203036418 @default.
- W2024797977 hasConcept C2776925733 @default.
- W2024797977 hasConcept C2780925461 @default.
- W2024797977 hasConcept C40767141 @default.
- W2024797977 hasConcept C43617362 @default.
- W2024797977 hasConcept C501593827 @default.
- W2024797977 hasConcept C547475151 @default.
- W2024797977 hasConcept C55493867 @default.
- W2024797977 hasConcept C58121356 @default.
- W2024797977 hasConcept C8010536 @default.
- W2024797977 hasConcept C86803240 @default.
- W2024797977 hasConceptScore W2024797977C104292427 @default.
- W2024797977 hasConceptScore W2024797977C104317684 @default.
- W2024797977 hasConceptScore W2024797977C113196181 @default.
- W2024797977 hasConceptScore W2024797977C13965031 @default.
- W2024797977 hasConceptScore W2024797977C153911025 @default.
- W2024797977 hasConceptScore W2024797977C178790620 @default.
- W2024797977 hasConceptScore W2024797977C181199279 @default.
- W2024797977 hasConceptScore W2024797977C185592680 @default.
- W2024797977 hasConceptScore W2024797977C203036418 @default.
- W2024797977 hasConceptScore W2024797977C2776925733 @default.
- W2024797977 hasConceptScore W2024797977C2780925461 @default.
- W2024797977 hasConceptScore W2024797977C40767141 @default.
- W2024797977 hasConceptScore W2024797977C43617362 @default.
- W2024797977 hasConceptScore W2024797977C501593827 @default.
- W2024797977 hasConceptScore W2024797977C547475151 @default.
- W2024797977 hasConceptScore W2024797977C55493867 @default.
- W2024797977 hasConceptScore W2024797977C58121356 @default.
- W2024797977 hasConceptScore W2024797977C8010536 @default.
- W2024797977 hasConceptScore W2024797977C86803240 @default.
- W2024797977 hasIssue "3" @default.
- W2024797977 hasLocation W20247979771 @default.
- W2024797977 hasLocation W20247979772 @default.
- W2024797977 hasOpenAccess W2024797977 @default.
- W2024797977 hasPrimaryLocation W20247979771 @default.
- W2024797977 hasRelatedWork W1499066748 @default.
- W2024797977 hasRelatedWork W1503226415 @default.
- W2024797977 hasRelatedWork W1987662354 @default.
- W2024797977 hasRelatedWork W1996629794 @default.
- W2024797977 hasRelatedWork W1999490032 @default.
- W2024797977 hasRelatedWork W2068766648 @default.
- W2024797977 hasRelatedWork W2083386540 @default.
- W2024797977 hasRelatedWork W2114087515 @default.
- W2024797977 hasRelatedWork W2350869260 @default.
- W2024797977 hasRelatedWork W2364400598 @default.
- W2024797977 hasVolume "57" @default.
- W2024797977 isParatext "false" @default.
- W2024797977 isRetracted "false" @default.
- W2024797977 magId "2024797977" @default.
- W2024797977 workType "article" @default.