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- W2024798314 abstract "A synthetic gene encoding the peptide sequence for the low molecular weight (Mr ∼ 9600 Da) high-potential iron protein (HiPIP) from the photosynthetic bacterium Chromatium vinosum has been constructed by shotgun ligation of twelve complimentary oligonudeotides varying in size from 42-mers to 48-mers. After cloning the gene into a pET-21d(+) vector, expression of holoprotein in yields of 35 mg/liter of culture was obtained following induction with isopropyl-β-D-thiogalactoside (IPTG). The recombinant protein was characterized by electronic absorption, 1H NMR, electrochemistry, N-terminal sequencing and amino acid analysis. This is the first example of the expression of a high potential ferredoxin containing a fully constituted [Fe4S4] cluster." @default.
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- W2024798314 date "1993-12-01" @default.
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- W2024798314 title "Synthesis, Cloning and Expression of a Synthetic Gene for High Potential Iron Protein from Chromatium vinosum" @default.
- W2024798314 doi "https://doi.org/10.1006/bbrc.1993.2626" @default.
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