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- W2024800421 abstract "The chlorocruorin of the marine polychaete Eudistylia vancouveri has a molecular weight of 3.1 · 106 and a sedimentation coefficient (s020, w) of about 57 S at pH 8.0 in the presence of 0.01 M Mg2+. The quaternary structure of this pigment is unaffected by pH between 6.0 and 11.5 in the presence of 0.01 M Mg2+ whereas in 0.01 M EDTA, the pigment begins to dissociate above pH 9.0 into smaller submultiples. The chlorocruorin can be converted into subunits with molecular weights of about 14 000–15 000 and 30 000 as determined by sodium dodecyl sulfate-gel electrophoresis and 14 000–15 000 as measured by gel chromatography of the carboxymethylated derivative in 8 M urea, 0.1 M 2-mercaptoethanol, or by sedimentation equilibrium in 6 M guanidine · HCl and 0.1 M 2-mercaptoethanol. The pigment contains 0.212 ± 0.008% iron corresponding to 1 g atom iron per 26 300 g chlorocruorin. The amino acid composition of this pigment is reported. The subunit structure of Eudistylia chlorocruorin and the polymeric annelid hemoglobins are similar in many respects." @default.
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- W2024800421 date "1975-08-01" @default.
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- W2024800421 title "Molecular weight of Eudistylia vancouveri chlorocruorin and its subunits" @default.
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- W2024800421 doi "https://doi.org/10.1016/0005-2795(75)90185-3" @default.
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