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• W2024840111 abstract "A significant fraction of the nuclear DNA of all eukaryotes is occupied by simple sequence repeats (SSRs) or microsatellites. This type of sequence has sparked great interest as a means of studying genetic variation, linkage mapping, gene tagging and evolution. Although SSRs at different positions in a gene help determine the regulation of expression and the function of the protein produced, little attention has been paid to the chromosomal organisation and distribution of these sequences, even in model species. This review discusses the main achievements in the characterisation of long-range SSR organisation in the chromosomes of <i>Triticum aestivum</i> L., <i>Secale cereale</i> L., and <i>Hordeum vulgare</i> L. (all members of Triticeae). We have detected SSRs using an improved FISH technique based on the random primer labelling of synthetic oligonucleotides (15–24 bases) in multi-colour experiments. Detailed information on the presence and distribution of AC, AG and all the possible classes of trinucleotide repeats has been acquired. These data have revealed the motif-dependent and non-random chromosome distributions of SSRs in the different genomes, and allowed the correlation of particular SSRs with chromosome areas characterised by specific features (e.g., heterochromatin, euchromatin and centromeres) in all three species. The present review provides a detailed comparative study of the distribution of these SSRs in each of the seven chromosomes of the genomes A, B and D of wheat, H of barley and R of rye. The importance of SSRs in plant breeding and their possible role in chromosome structure, function and evolution is discussed." @default.
• W2024840111 created "2016-06-24" @default.
• W2024840111 creator A5062200901 @default.
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• W2024840111 date "2008-01-01" @default.
• W2024840111 modified "2023-10-14" @default.
• W2024840111 title "Physical organisation of simple sequence repeats (SSRs) in Triticeae: structural, functional and evolutionary implications" @default.
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• W2024840111 doi "https://doi.org/10.1159/000121069" @default.
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