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• W2024851055 abstract "To investigate the efficient transduction of tumor cells which remains a major limitation of cancer gene therapy.. In this study, we tested whether treatment with antimetabolic drugs 5-FU and hydroxyurea (HU) could improve adenovirus-mediated gene expression in tumor cells. We found that 5-FU and HU treatment significantly increased β-gal activity in adenovirus (Ad.CMVBG)-infected human colon carcinoma (LoVo) and hepatocellular carcinoma (SMMC7721) cells in a dose- and time-dependent manner. These increases were maximized at 5.01±0.42-fold and 3.32±0.32-fold for 5-FU (50 μM), and at 6.60±0.50-fold and 4.82±0.43-fold for HU (5 mM) treatment, respectively, after 48 h infection. Transient increases in viral uptake, determined by real-time PCR for viral DNA content and by confocal microscopy for viral particles, were observed in 5-FU or HU-treated cells that partially contribute to the overall increases of β-gal expression. Moreover, mRNA levels for the β-gal gene in infected cells were significantly increased in both LoVo and SMMC7721 cells by 5-FU and HU treatment in contrast to the inhibition of viral DNA replication and the unchanged mRNA levels for α-actin gene. The induction appeared to be the result of enhanced transcription since β-gal mRNA half-life was not affected by drug treatment. However, similar induction was not detected in CMV-β-gal-expressing stable cells, suggesting that an adenovirus-associated mechanism might be involved in this induction. Our findings suggest that it may be possible to improve tumor cell transduction by adenovirus using chemotherapy." @default.
• W2024851055 created "2016-06-24" @default.
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• W2024851055 date "2004-11-09" @default.
• W2024851055 modified "2023-09-26" @default.
• W2024851055 title "5-fluorouracil and hydroxyurea enhance adenovirus-mediated transgene expression in colon and hepatocellular carcinoma cells" @default.
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• W2024851055 doi "https://doi.org/10.1007/s00432-004-0608-1" @default.
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