FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2024851063> ?p ?o ?g. }

• W2024851063 endingPage "1659" @default.
• W2024851063 startingPage "1650" @default.
• W2024851063 abstract "Abstract Demand for increasingly complex post‐translationally modified proteins, such as monoclonal antibodies (mAbs), necessitates the use of mammalian hosts for production. The focus of this article is a continuous centrifugal bioreactor (CCBR) capable of increasing volumetric productivity for mAb production through high density hybridoma culture, exceeding 10 8 cells/mL. At these extreme densities, environmental conditions such as substrate and inhibitor concentrations rapidly change dramatically affecting the growth rate. The development of a kinetic model predicting glucose, mAb, lactate, and ammonium concentrations based on dilution rate and cell density is shown in this article. Additionally, it is found that pH affects both growth rate and viability, and a range of 6.9–7.4 is needed to maintain growth rate above 90% of the maximum. Modeling shows that operating an 11.4 mL CCBR inoculated with 2.0 × 10 7 cells/mL at a dilution rate of 1.3 h –1 , results in a predicted growth rate 82% of the maximum value. At the same dilution rate increasing density to 6.0 × 10 7 cells/mL decreases the predicted growth rate to 60% of the maximum; however, by increasing dilution rate to 6.1 h −1 the growth rate can be increased to 86% of the maximum. Using the kinetic model developed in this research, the concentration of glucose, mAb, lactate, and ammonium are all predicted within 13% of experimental results. This model and an understanding of how RPM impacts cell retention serve as valuable tools for maintaining high density CCBR cultures, ensuring maximum growth associated mAb production rates. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2009" @default.
• W2024851063 created "2016-06-24" @default.
• W2024851063 creator A5038106252 @default.
• W2024851063 creator A5039088246 @default.
• W2024851063 creator A5059037543 @default.
• W2024851063 creator A5081037692 @default.
• W2024851063 date "2009-10-05" @default.
• W2024851063 modified "2023-10-18" @default.
• W2024851063 title "Kinetic simulation of a centrifugal bioreactor for high population density hybridoma culture" @default.
• W2024851063 cites W1899319846 @default.
• W2024851063 cites W1964219106 @default.
• W2024851063 cites W1964696099 @default.
• W2024851063 cites W1972158451 @default.
• W2024851063 cites W1973972095 @default.
• W2024851063 cites W1977404096 @default.
• W2024851063 cites W1978321106 @default.
• W2024851063 cites W1986316867 @default.
• W2024851063 cites W1989420467 @default.
• W2024851063 cites W1990767767 @default.
• W2024851063 cites W1994142817 @default.
• W2024851063 cites W2004241087 @default.
• W2024851063 cites W2008678023 @default.
• W2024851063 cites W2009022524 @default.
• W2024851063 cites W2014618083 @default.
• W2024851063 cites W2021351846 @default.
• W2024851063 cites W2025138920 @default.
• W2024851063 cites W2033266068 @default.
• W2024851063 cites W2038324009 @default.
• W2024851063 cites W2038613649 @default.
• W2024851063 cites W2042716641 @default.
• W2024851063 cites W2047675204 @default.
• W2024851063 cites W2048398569 @default.
• W2024851063 cites W2050064457 @default.
• W2024851063 cites W2050921552 @default.
• W2024851063 cites W2054814854 @default.
• W2024851063 cites W2056368762 @default.
• W2024851063 cites W2057540854 @default.
• W2024851063 cites W2066983053 @default.
• W2024851063 cites W2075907729 @default.
• W2024851063 cites W2100438295 @default.
• W2024851063 cites W2117727600 @default.
• W2024851063 cites W2121811155 @default.
• W2024851063 cites W2126966500 @default.
• W2024851063 cites W2145283521 @default.
• W2024851063 cites W2147910809 @default.
• W2024851063 cites W2149826965 @default.
• W2024851063 cites W2151142303 @default.
• W2024851063 cites W2166135558 @default.
• W2024851063 cites W2417506204 @default.
• W2024851063 cites W962985142 @default.
• W2024851063 cites W9955020 @default.
• W2024851063 doi "https://doi.org/10.1002/btpr.240" @default.
• W2024851063 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/2796705" @default.
• W2024851063 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/19806634" @default.
• W2024851063 hasPublicationYear "2009" @default.
• W2024851063 type Work @default.
• W2024851063 sameAs 2024851063 @default.
• W2024851063 citedByCount "5" @default.
• W2024851063 countsByYear W20248510632016 @default.
• W2024851063 countsByYear W20248510632023 @default.
• W2024851063 crossrefType "journal-article" @default.
• W2024851063 hasAuthorship W2024851063A5038106252 @default.
• W2024851063 hasAuthorship W2024851063A5039088246 @default.
• W2024851063 hasAuthorship W2024851063A5059037543 @default.
• W2024851063 hasAuthorship W2024851063A5081037692 @default.
• W2024851063 hasBestOaLocation W20248510632 @default.
• W2024851063 hasConcept C121332964 @default.
• W2024851063 hasConcept C139719470 @default.
• W2024851063 hasConcept C159654299 @default.
• W2024851063 hasConcept C162324750 @default.
• W2024851063 hasConcept C168170006 @default.
• W2024851063 hasConcept C178790620 @default.
• W2024851063 hasConcept C185592680 @default.
• W2024851063 hasConcept C18903297 @default.
• W2024851063 hasConcept C203014093 @default.
• W2024851063 hasConcept C204983608 @default.
• W2024851063 hasConcept C2524010 @default.
• W2024851063 hasConcept C25642318 @default.
• W2024851063 hasConcept C2777289219 @default.
• W2024851063 hasConcept C2778312390 @default.
• W2024851063 hasConcept C2779870022 @default.
• W2024851063 hasConcept C33923547 @default.
• W2024851063 hasConcept C43617362 @default.
• W2024851063 hasConcept C542903549 @default.
• W2024851063 hasConcept C55493867 @default.
• W2024851063 hasConcept C59822182 @default.
• W2024851063 hasConcept C86803240 @default.
• W2024851063 hasConcept C96618451 @default.
• W2024851063 hasConcept C97355855 @default.
• W2024851063 hasConceptScore W2024851063C121332964 @default.
• W2024851063 hasConceptScore W2024851063C139719470 @default.
• W2024851063 hasConceptScore W2024851063C159654299 @default.
• W2024851063 hasConceptScore W2024851063C162324750 @default.
• W2024851063 hasConceptScore W2024851063C168170006 @default.
• W2024851063 hasConceptScore W2024851063C178790620 @default.
• W2024851063 hasConceptScore W2024851063C185592680 @default.
• W2024851063 hasConceptScore W2024851063C18903297 @default.
• W2024851063 hasConceptScore W2024851063C203014093 @default.

• next