FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2024852868> ?p ?o ?g. }

• W2024852868 endingPage "2151" @default.
• W2024852868 startingPage "2148" @default.
• W2024852868 abstract "Escherichia coli O157:H7 (O157) is a rare serotype of E. coli that produces large quantities of a powerful toxin that can severely damage the lining of the intestine and induce extreme diarrhea, hemorrhagic colitis, and kidney damage. People are commonly infected by food or water; because bacteria can live asymptomatically in healthy ruminant mammals, meats can become contaminated during processing (1)(2). A rapid test to identify contaminated foodstuffs, water supplies, and infected persons would assist in reducing the risk of spreading this disease.Many assays have been developed for detection of O157 (3)(4)(5)(6). These methods are time-consuming, however, if they require bacterial culture and may be too complex and costly for use in routine analysis. Rapid confirmation PCR assays for O157 have recently been developed (7)(8), but inhibition of PCR by humic compounds present in complex matrices such as food and surface waters can lead to difficulties such as false-negative results. To address these shortcomings, we developed a rapid, specific proof-of-principle assay for the detection of E. coli O157:H7 with Resonant Acoustic Profiling™ (RAP) technology (9).We obtained heat-inactivated E. coli O157:H7 cells from Kirkegaard and Perry Laboratory. Five clinical E. coli isolates provided by Dr Derek Brown (Addenbrookes, Cambridge, UK) and an American Type Culture Collection E. coli 25922 were used as negative controls. Five of these isolates were live bacteria, and heat-inactivated E. coli 170044 was used as a killed control. Affinity-purified goat anti-O157 polyclonal antibodies (pAbs) were obtained from Kirkegaard and Perry Laboratory. Anti-O157 pAb was isolated from a pooled serum from goats immunized with heat-killed whole cells of E. coli O157:H7. The lyophilized anti-O157 antibody was reconstituted with 0.3 mol/L sodium phosphate buffer (0.1 mol/L NaH2PO4, 0.2 mol/L Na2HPO …" @default.
• W2024852868 created "2016-06-24" @default.
• W2024852868 creator A5021936835 @default.
• W2024852868 creator A5090230661 @default.
• W2024852868 date "2006-11-01" @default.
• W2024852868 modified "2023-10-14" @default.
• W2024852868 title "Real-Time Label-Free Acoustic Technology for Rapid Detection of Escherichia coli O157:H7" @default.
• W2024852868 cites W1982413694 @default.
• W2024852868 cites W1986752852 @default.
• W2024852868 cites W2026723159 @default.
• W2024852868 cites W2031197500 @default.
• W2024852868 cites W2041030091 @default.
• W2024852868 cites W2049985802 @default.
• W2024852868 cites W2055322845 @default.
• W2024852868 cites W2117880227 @default.
• W2024852868 cites W2126969790 @default.
• W2024852868 cites W2143077693 @default.
• W2024852868 doi "https://doi.org/10.1373/clinchem.2006.072652" @default.
• W2024852868 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18050522" @default.
• W2024852868 hasPublicationYear "2006" @default.
• W2024852868 type Work @default.
• W2024852868 sameAs 2024852868 @default.
• W2024852868 citedByCount "9" @default.
• W2024852868 countsByYear W20248528682012 @default.
• W2024852868 crossrefType "journal-article" @default.
• W2024852868 hasAuthorship W2024852868A5021936835 @default.
• W2024852868 hasAuthorship W2024852868A5090230661 @default.
• W2024852868 hasBestOaLocation W20248528681 @default.
• W2024852868 hasConcept C104317684 @default.
• W2024852868 hasConcept C185592680 @default.
• W2024852868 hasConcept C547475151 @default.
• W2024852868 hasConcept C55493867 @default.
• W2024852868 hasConcept C86803240 @default.
• W2024852868 hasConcept C89423630 @default.
• W2024852868 hasConceptScore W2024852868C104317684 @default.
• W2024852868 hasConceptScore W2024852868C185592680 @default.
• W2024852868 hasConceptScore W2024852868C547475151 @default.
• W2024852868 hasConceptScore W2024852868C55493867 @default.
• W2024852868 hasConceptScore W2024852868C86803240 @default.
• W2024852868 hasConceptScore W2024852868C89423630 @default.
• W2024852868 hasIssue "11" @default.
• W2024852868 hasLocation W20248528681 @default.
• W2024852868 hasLocation W20248528682 @default.
• W2024852868 hasOpenAccess W2024852868 @default.
• W2024852868 hasPrimaryLocation W20248528681 @default.
• W2024852868 hasRelatedWork W1935994699 @default.
• W2024852868 hasRelatedWork W2360987025 @default.
• W2024852868 hasRelatedWork W2376819606 @default.
• W2024852868 hasRelatedWork W2382269147 @default.
• W2024852868 hasRelatedWork W2387178301 @default.
• W2024852868 hasRelatedWork W2785993273 @default.
• W2024852868 hasRelatedWork W2992955585 @default.
• W2024852868 hasRelatedWork W3144733943 @default.
• W2024852868 hasRelatedWork W3184323118 @default.
• W2024852868 hasRelatedWork W36834100 @default.
• W2024852868 hasVolume "52" @default.
• W2024852868 isParatext "false" @default.
• W2024852868 isRetracted "false" @default.
• W2024852868 magId "2024852868" @default.
• W2024852868 workType "article" @default.