FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2024876937> ?p ?o ?g. }

• W2024876937 endingPage "2573" @default.
• W2024876937 startingPage "2569" @default.
• W2024876937 abstract "The family of matrix metalloproteinases is a family of closely related enzymes that play an important role in physiological and pathological processes of matrix degradation. The most distinctive characteristic of interstitial collagenases (fibroblast and neutrophil collagenases) is their ability to cleave interstitial collagens at a single peptide bond; however, the precise region of the enzyme responsible for this substrate specificity remains to be defined. To address this question, we generated truncated mutants of neutrophil collagenase with various deletions in the COOH-terminal domain and chimeric molecules between neutrophil collagenase and stromelysin and assayed the expressed enzymes against type I collagen and the general substrate, casein. Our data suggest that substrate specificity for interstitial collagen is determined by a 16-aa sequence in the COOH-terminal domain of neutrophil collagenase and is influenced by the integrity of a disulfide-defined loop at the COOH terminus for maximal activity. It was found that a relatively large region of 62-aa residues influenced the relative efficiency of collagenolytic activity. In addition to the region that conferred this specificity, a site at the COOH side of the presumptive zinc-binding locus was found to be necessary for general catalytic activity. Mutation of a critical aspartic residue at position 253 within this area resulted in complete loss of proteolytic activity, suggesting that Asp-253 might function as one of the ligands for divalent cations, which are essential for enzymatic activity." @default.
• W2024876937 created "2016-06-24" @default.
• W2024876937 creator A5023635229 @default.
• W2024876937 creator A5033672633 @default.
• W2024876937 creator A5041158418 @default.
• W2024876937 creator A5077084566 @default.
• W2024876937 creator A5082174745 @default.
• W2024876937 date "1993-04-01" @default.
• W2024876937 modified "2023-10-03" @default.
• W2024876937 title "Structure-function relationship of human neutrophil collagenase: identification of regions responsible for substrate specificity and general proteinase activity." @default.
• W2024876937 cites W1491598928 @default.
• W2024876937 cites W1507420346 @default.
• W2024876937 cites W1521619375 @default.
• W2024876937 cites W1524239466 @default.
• W2024876937 cites W1535467323 @default.
• W2024876937 cites W1535820474 @default.
• W2024876937 cites W1546229037 @default.
• W2024876937 cites W1559508265 @default.
• W2024876937 cites W1567487285 @default.
• W2024876937 cites W1577422435 @default.
• W2024876937 cites W1583304743 @default.
• W2024876937 cites W1583907090 @default.
• W2024876937 cites W1598340803 @default.
• W2024876937 cites W1628604166 @default.
• W2024876937 cites W1669342624 @default.
• W2024876937 cites W176943133 @default.
• W2024876937 cites W1984837363 @default.
• W2024876937 cites W2017185391 @default.
• W2024876937 cites W2021116136 @default.
• W2024876937 cites W2022676856 @default.
• W2024876937 cites W2027054547 @default.
• W2024876937 cites W2028727659 @default.
• W2024876937 cites W2040089477 @default.
• W2024876937 cites W2042492216 @default.
• W2024876937 cites W2043254359 @default.
• W2024876937 cites W2071072558 @default.
• W2024876937 cites W2083715055 @default.
• W2024876937 cites W2155925117 @default.
• W2024876937 cites W2167709602 @default.
• W2024876937 cites W2176433489 @default.
• W2024876937 cites W238232679 @default.
• W2024876937 cites W2415881300 @default.
• W2024876937 doi "https://doi.org/10.1073/pnas.90.7.2569" @default.
• W2024876937 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/46136" @default.
• W2024876937 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8464863" @default.
• W2024876937 hasPublicationYear "1993" @default.
• W2024876937 type Work @default.
• W2024876937 sameAs 2024876937 @default.
• W2024876937 citedByCount "118" @default.
• W2024876937 countsByYear W20248769372012 @default.
• W2024876937 countsByYear W20248769372013 @default.
• W2024876937 countsByYear W20248769372014 @default.
• W2024876937 countsByYear W20248769372015 @default.
• W2024876937 countsByYear W20248769372016 @default.
• W2024876937 countsByYear W20248769372017 @default.
• W2024876937 countsByYear W20248769372018 @default.
• W2024876937 countsByYear W20248769372019 @default.
• W2024876937 countsByYear W20248769372021 @default.
• W2024876937 crossrefType "journal-article" @default.
• W2024876937 hasAuthorship W2024876937A5023635229 @default.
• W2024876937 hasAuthorship W2024876937A5033672633 @default.
• W2024876937 hasAuthorship W2024876937A5041158418 @default.
• W2024876937 hasAuthorship W2024876937A5077084566 @default.
• W2024876937 hasAuthorship W2024876937A5082174745 @default.
• W2024876937 hasBestOaLocation W20248769371 @default.
• W2024876937 hasConcept C104317684 @default.
• W2024876937 hasConcept C109523444 @default.
• W2024876937 hasConcept C143065580 @default.
• W2024876937 hasConcept C153911025 @default.
• W2024876937 hasConcept C181199279 @default.
• W2024876937 hasConcept C182220744 @default.
• W2024876937 hasConcept C185592680 @default.
• W2024876937 hasConcept C2776747403 @default.
• W2024876937 hasConcept C2778394429 @default.
• W2024876937 hasConcept C2781307694 @default.
• W2024876937 hasConcept C55493867 @default.
• W2024876937 hasConcept C86803240 @default.
• W2024876937 hasConceptScore W2024876937C104317684 @default.
• W2024876937 hasConceptScore W2024876937C109523444 @default.
• W2024876937 hasConceptScore W2024876937C143065580 @default.
• W2024876937 hasConceptScore W2024876937C153911025 @default.
• W2024876937 hasConceptScore W2024876937C181199279 @default.
• W2024876937 hasConceptScore W2024876937C182220744 @default.
• W2024876937 hasConceptScore W2024876937C185592680 @default.
• W2024876937 hasConceptScore W2024876937C2776747403 @default.
• W2024876937 hasConceptScore W2024876937C2778394429 @default.
• W2024876937 hasConceptScore W2024876937C2781307694 @default.
• W2024876937 hasConceptScore W2024876937C55493867 @default.
• W2024876937 hasConceptScore W2024876937C86803240 @default.
• W2024876937 hasIssue "7" @default.
• W2024876937 hasLocation W20248769371 @default.
• W2024876937 hasLocation W20248769372 @default.
• W2024876937 hasLocation W20248769373 @default.
• W2024876937 hasLocation W20248769374 @default.
• W2024876937 hasOpenAccess W2024876937 @default.
• W2024876937 hasPrimaryLocation W20248769371 @default.
• W2024876937 hasRelatedWork W1649452503 @default.
• W2024876937 hasRelatedWork W2015031004 @default.

• next