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• W2024881218 startingPage "129" @default.
• W2024881218 abstract "Expression plasmids were constructed containing chemically synthesized human epidermal growth factor (EGF) gene fused in a frame to a leader sequence of human interleukin-2 (IL-2) gene under the control of a viral promoter. COS7 cells transfected with the plasmids synthesized and secreted EGF. Transfection of mouse A9 cells or BALB/3T3 clone A31 cells with the plasmids permitted the isolation of cell lines secreting the product which showed EGF activity. In particular, A31 transformed cells secreting human EGF grew well even in a medium containing a minimal level of serum. Using similar vectors having IgE cDNA (C2-C4) in place of EGF gene, a human IgE Fc fragment was also produced and secreted in mouse cells. These results show that heterologous leader sequences are useful for the expression and secretion of proteins whose genes lack leader sequences." @default.
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• W2024881218 date "1988-01-01" @default.
• W2024881218 modified "2023-09-27" @default.
• W2024881218 title "Secretion of human EGF and IgE in mammalian cells by recombinant DNA techniques; Use of a IL-2 leader sequence." @default.
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• W2024881218 doi "https://doi.org/10.1247/csf.13.129" @default.
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