FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2024881804> ?p ?o ?g. }

• W2024881804 endingPage "1646" @default.
• W2024881804 startingPage "1641" @default.
• W2024881804 abstract "Progress in prostate cancer research has been hindered by the lack of well characterized, immortalized, human prostatic epithelial cell lines that express markers of normal prostatic epithelial cells and mimic normal growth and differentiation responses to androgens. The objectives of this study were to: (i) establish immortalized cell lines from non-neoplastic, adult human prostatic epithelium using adenovirus-12/simian virus-40 (Ad12-SV40) hybrid virus; (ii) establish their prostatic epithelial origin; (iii) demonstrate androgen responsiveness; and (iv) examine response to growth factors. Primary epithelial cell cultures derived from a non-neoplastic, adult human prostate were infected with the Ad12-SV40 virus. Several immortalized clones were isolated. Single cell cloning of one clone, free of cytopathic effects, gave rise to the PWr-1E cell line. An immortalized cell line PWR-1E, which expresses many characteristics of normal prostatic epithelial cells was established. Immunostaining showed that cells express cytokeratins 8 and 18 normally expressed by differentiated, secretory prostatic epithelial cells. The most remarkable characteristics of PWR-1E cells are growth stimulation, increased expression of androgen receptor and induction of prostate specific antigen (PSA) expression in response to androgens, which indisputably establish their prostatic epithelial origin. They are positive for SV40 large-T antigen and show strong nuclear staining for p53. Cells from passages 23 and 40 were not tumorigenic in nude mice even when co-injected with Matrigel. They grow in a serum-free defined medium and respond to EGF, bFGF and TGF-beta. Passage 42-cells showed a human male (XY), hyperdiploid karyotype. The PWR-1E cell line is the only known Ad12-SV40-immortalized human prostatic epithelial cell line. PWR-1E cells can be used to study (i) the etiology and the multistep process of carcinogenesis and tumor progression in the human prostate; (ii) normal prostate physiology and differentiation; and (iii) potential prostate cancer chemopreventive agents." @default.
• W2024881804 created "2016-06-24" @default.
• W2024881804 creator A5020987510 @default.
• W2024881804 creator A5041645294 @default.
• W2024881804 creator A5044156119 @default.
• W2024881804 creator A5054622398 @default.
• W2024881804 creator A5069412890 @default.
• W2024881804 creator A5087872496 @default.
• W2024881804 date "1996-01-01" @default.
• W2024881804 modified "2023-09-24" @default.
• W2024881804 title "Prostate specific antigen and androgen receptor induction and characterization of an immortalized adult human prostatic epithelial cell line" @default.
• W2024881804 doi "https://doi.org/10.1093/carcin/17.8.1641" @default.
• W2024881804 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8761420" @default.
• W2024881804 hasPublicationYear "1996" @default.
• W2024881804 type Work @default.
• W2024881804 sameAs 2024881804 @default.
• W2024881804 citedByCount "56" @default.
• W2024881804 countsByYear W20248818042012 @default.
• W2024881804 countsByYear W20248818042013 @default.
• W2024881804 countsByYear W20248818042015 @default.
• W2024881804 countsByYear W20248818042016 @default.
• W2024881804 countsByYear W20248818042017 @default.
• W2024881804 countsByYear W20248818042018 @default.
• W2024881804 countsByYear W20248818042022 @default.
• W2024881804 countsByYear W20248818042023 @default.
• W2024881804 crossrefType "journal-article" @default.
• W2024881804 hasAuthorship W2024881804A5020987510 @default.
• W2024881804 hasAuthorship W2024881804A5041645294 @default.
• W2024881804 hasAuthorship W2024881804A5044156119 @default.
• W2024881804 hasAuthorship W2024881804A5054622398 @default.
• W2024881804 hasAuthorship W2024881804A5069412890 @default.
• W2024881804 hasAuthorship W2024881804A5087872496 @default.
• W2024881804 hasBestOaLocation W20248818041 @default.
• W2024881804 hasConcept C121608353 @default.
• W2024881804 hasConcept C126322002 @default.
• W2024881804 hasConcept C134018914 @default.
• W2024881804 hasConcept C147483822 @default.
• W2024881804 hasConcept C203014093 @default.
• W2024881804 hasConcept C2775971809 @default.
• W2024881804 hasConcept C2776235491 @default.
• W2024881804 hasConcept C2777911890 @default.
• W2024881804 hasConcept C2778608917 @default.
• W2024881804 hasConcept C2781406297 @default.
• W2024881804 hasConcept C2993273003 @default.
• W2024881804 hasConcept C502942594 @default.
• W2024881804 hasConcept C529295009 @default.
• W2024881804 hasConcept C54009773 @default.
• W2024881804 hasConcept C54355233 @default.
• W2024881804 hasConcept C552990157 @default.
• W2024881804 hasConcept C555283112 @default.
• W2024881804 hasConcept C6045943 @default.
• W2024881804 hasConcept C71315377 @default.
• W2024881804 hasConcept C71924100 @default.
• W2024881804 hasConcept C81089528 @default.
• W2024881804 hasConcept C81885089 @default.
• W2024881804 hasConcept C86803240 @default.
• W2024881804 hasConceptScore W2024881804C121608353 @default.
• W2024881804 hasConceptScore W2024881804C126322002 @default.
• W2024881804 hasConceptScore W2024881804C134018914 @default.
• W2024881804 hasConceptScore W2024881804C147483822 @default.
• W2024881804 hasConceptScore W2024881804C203014093 @default.
• W2024881804 hasConceptScore W2024881804C2775971809 @default.
• W2024881804 hasConceptScore W2024881804C2776235491 @default.
• W2024881804 hasConceptScore W2024881804C2777911890 @default.
• W2024881804 hasConceptScore W2024881804C2778608917 @default.
• W2024881804 hasConceptScore W2024881804C2781406297 @default.
• W2024881804 hasConceptScore W2024881804C2993273003 @default.
• W2024881804 hasConceptScore W2024881804C502942594 @default.
• W2024881804 hasConceptScore W2024881804C529295009 @default.
• W2024881804 hasConceptScore W2024881804C54009773 @default.
• W2024881804 hasConceptScore W2024881804C54355233 @default.
• W2024881804 hasConceptScore W2024881804C552990157 @default.
• W2024881804 hasConceptScore W2024881804C555283112 @default.
• W2024881804 hasConceptScore W2024881804C6045943 @default.
• W2024881804 hasConceptScore W2024881804C71315377 @default.
• W2024881804 hasConceptScore W2024881804C71924100 @default.
• W2024881804 hasConceptScore W2024881804C81089528 @default.
• W2024881804 hasConceptScore W2024881804C81885089 @default.
• W2024881804 hasConceptScore W2024881804C86803240 @default.
• W2024881804 hasIssue "8" @default.
• W2024881804 hasLocation W20248818041 @default.
• W2024881804 hasLocation W20248818042 @default.
• W2024881804 hasOpenAccess W2024881804 @default.
• W2024881804 hasPrimaryLocation W20248818041 @default.
• W2024881804 hasRelatedWork W1525023012 @default.
• W2024881804 hasRelatedWork W1892390431 @default.
• W2024881804 hasRelatedWork W2024881804 @default.
• W2024881804 hasRelatedWork W2073455911 @default.
• W2024881804 hasRelatedWork W2083050351 @default.
• W2024881804 hasRelatedWork W2110977091 @default.
• W2024881804 hasRelatedWork W2379116031 @default.
• W2024881804 hasRelatedWork W2384842966 @default.
• W2024881804 hasRelatedWork W25534225 @default.
• W2024881804 hasRelatedWork W2907269396 @default.
• W2024881804 hasVolume "17" @default.
• W2024881804 isParatext "false" @default.
• W2024881804 isRetracted "false" @default.
• W2024881804 magId "2024881804" @default.

• next