FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2024883517> ?p ?o ?g. }

• W2024883517 endingPage "592" @default.
• W2024883517 startingPage "583" @default.
• W2024883517 abstract "No AccessJournal of UrologyInvestigative Urology1 Aug 2014Bladder Cancer Exosomes Contain EDIL-3/Del1 and Facilitate Cancer Progression Carla J. Beckham, Jayme Olsen, Peng-Nien Yin, Chia-Hao Wu, Huei-Ju Ting, Fred K. Hagen, Emelian Scosyrev, Edward M. Messing, and Yi-Fen Lee Carla J. BeckhamCarla J. Beckham Department of Urology, University of Rochester, Rochester, New York More articles by this author , Jayme OlsenJayme Olsen Department of Genetics, University of Rochester, Rochester, New York More articles by this author , Peng-Nien YinPeng-Nien Yin Department of Urology, University of Rochester, Rochester, New York More articles by this author , Chia-Hao WuChia-Hao Wu Department of Urology, University of Rochester, Rochester, New York More articles by this author , Huei-Ju TingHuei-Ju Ting Department of Biological Science and Technology, National University of Tainan, Taiwan, Republic of China More articles by this author , Fred K. HagenFred K. Hagen Department of Biochemistry and Biophysics Proteomics Center, University of Rochester, Rochester, New York More articles by this author , Emelian ScosyrevEmelian Scosyrev Department of Urology, University of Rochester, Rochester, New York More articles by this author , Edward M. MessingEdward M. Messing Department of Urology, University of Rochester, Rochester, New York More articles by this author , and Yi-Fen LeeYi-Fen Lee Department of Urology, University of Rochester, Rochester, New York More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2014.02.035AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract Purpose: High grade bladder cancer is an extremely aggressive malignancy associated with high rates of morbidity and mortality. Understanding how exosomes may affect bladder cancer progression could reveal novel therapeutic targets. Materials and Methods: Exosomes derived from human bladder cancer cell lines and the urine of patients with high grade bladder cancer were assessed for the ability to promote cancer progression in standard assays. Exosomes purified from the high grade bladder cancer cell line TCC-SUP and the nonmalignant urothelial cell line SV-HUC were submitted for mass spectrometry analysis. EDIL-3 was identified and selected for further analysis. Western blot was done to determine EDIL-3 levels in urinary exosomes from patients with high grade bladder cancer. shRNA gene knockdown and recombinant EDIL-3 were applied to study EDIL-3 function. Results: Exosomes isolated from high grade bladder cancer cells and the urine of patients with high grade bladder cancer promoted angiogenesis and migration of bladder cancer cells and endothelial cells. We silenced EDIL-3 expression and found that shEDIL-3 exosomes did not facilitate angiogenesis, and urothelial and endothelial cell migration. Moreover, exosomes purified from the urine of patients with high grade bladder cancer contained significantly higher EDIL-3 levels than exosomes from the urine of healthy controls. EDIL-3 activated epidermal growth factor receptor signaling while blockade of epidermal growth factor receptor signaling abrogated this EDIL-3 induced bladder cell migration. Conclusions: Exosomes derived from the urine of patients with bladder cancer contains bioactive molecules such as EDIL-3. Identifying these components and their associated oncogenic pathways could lead to novel therapeutic targets and treatment strategies. References 1 : Exosomes/microvesicles as a mechanism of cell-to-cell communication. Kidney Int2010; 78: 838. Google Scholar 2 : Glioblastoma microvesicles transport RNA and proteins that promote tumour growth and provide diagnostic biomarkers. Nat Cell Biol2008; 10: 1470. Google Scholar 3 : Tumour microvesicles contain retrotransposon elements and amplified oncogene sequences. Nat Commun2011; 2: 180. Google Scholar 4 : Exosome-mediated transfer of mRNAs and microRNAs is a novel mechanism of genetic exchange between cells. Nat Cell Biol2007; 9: 654. Google Scholar 5 : Cancer exosomes trigger fibroblast to myofibroblast differentiation. Cancer Res2010; 70: 9621. Google Scholar 6 : Isolation and characterization of exosomes from cell culture supernatants and biological fluids. Curr Protoc Cell Biol2006; . chapt 3, unit 3, p 22. Google Scholar 7 : Urinary exosomes and proteomics. Mass Spectrom Rev2011; 30: 1185. Google Scholar 8 : Proteomics analysis of bladder cancer exosomes. Mol Cell Proteomics2010; 9: 1324. Google Scholar 9 : Collection, storage, preservation, and normalization of human urinary exosomes for biomarker discovery. Kidney Int2006; 69: 1471. Google Scholar 10 : Cancer cell-derived microvesicles induce transformation by transferring tissue transglutaminase and fibronectin to recipient cells. Proc Natl Acad Sci U S A2011; 108: 4852. Google Scholar 11 : Melanoma exosomes educate bone marrow progenitor cells toward a pro-metastatic phenotype through MET. Nat Med2012; 18: 883. Google Scholar 12 : Microfluidic isolation and transcriptome analysis of serum microvesicles. Lab Chip2010; 10: 505. Google Scholar 13 : The extracellular matrix protein Del1 induces apoptosis via its epidermal growth factor motif. Biochem Biophys Res Commun2010; 393: 757. Google Scholar 14 : Del-1, an endogenous leukocyte-endothelial adhesion inhibitor, limits inflammatory cell recruitment. Science2008; 322: 1101. Google Scholar 15 : The embryonic angiogenic factor Del1 accelerates tumor growth by enhancing vascular formation. Microvasc Res2002; 64: 148. Google Scholar 16 : High expression level of EDIL3 in HCC predicts poor prognosis of HCC patients. World J Gastroenterol2010; 16: 4611. Google Scholar 17 : Predicting ulcerative colitis-associated colorectal cancer using reverse-transcription polymerase chain reaction analysis. Clin Colorectal Cancer2011; 10: 134. Google Scholar 18 : Exosomes: composition, biogenesis and function. Nat Rev Immunol2002; 2: 569. Google Scholar 19 : In vitro scratch assay: a convenient and inexpensive method for analysis of cell migration in vitro. Nat Protoc2007; 2: 329. Google Scholar 20 : The chemoinvasion assay: a method to assess tumor and endothelial cell invasion and its modulation. Nat Protoc2007; 2: 504. Google Scholar 21 : Paracrine induction of endothelium by tumor exosomes. Lab Invest2009; 89: 1317. Google Scholar 22 : In vitro angiogenesis: endothelial cell tube formation on gelled basement membrane extract. Nat Protoc2010; 5: 628. Google Scholar 23 : The role of adhesion molecules, alpha v beta 3, alpha v beta 5 and their ligands in the tumor cell and endothelial cell adhesion. Eur J Cancer Prev2007; 16: 517. Google Scholar 24 : Neovascularization of ischemic tissues by gene delivery of the extracellular matrix protein Del-1. J Clin Invest2003; 112: 30. Google Scholar 25 : Epidermal growth factor-like repeats of thrombospondins activate phospholipase Cgamma and increase epithelial cell migration through indirect epidermal growth factor receptor activation. J Biol Chem2009; 284: 6389. Google Scholar 26 : Integrin alpha 2 beta 1-dependent EGF receptor activation at cell-cell contact sites. J Cell Sci2000; 113: 2139. Google Scholar 27 : A novel mechanism of PPAR gamma induction via EGFR signalling constitutes rational for combination therapy in bladder cancer. PLoS One2013; 8: e55997. Google Scholar 28 : Epidermal growth factor receptor-regulated human bladder cancer motility is in part a phosphatidylinositol 3-kinase-mediated process. Cell Growth Differ1998; 9: 919. Google Scholar 29 : Milk fat globule-EGF factor 8/lactadherin plays a crucial role in maintenance and repair of murine intestinal epithelium. J Clin Invest2007; 117: 3673. Google Scholar 30 : Milk fat globule-epidermal growth factor 8 is decreased in intestinal epithelium of ulcerative colitis patients and thereby causes increased apoptosis and impaired wound healing. Mol Med2012; 18: 497. Google Scholar © 2014 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetailsCited byAtala A (2019) Re: Bladder Cancer Extracellular Vesicles Drive Tumorigenesis by Inducing the Unfolded Protein Response in Endoplasmic Reticulum of Nonmalignant CellsJournal of Urology, VOL. 202, NO. 6, (1100-1101), Online publication date: 1-Dec-2019.Franzen C, Blackwell R, Foreman K, Kuo P, Flanigan R and Gupta G (2018) Urinary Exosomes: The Potential for Biomarker Utility, Intercellular Signaling and Therapeutics in Urological MalignancyJournal of Urology, VOL. 195, NO. 5, (1331-1339), Online publication date: 1-May-2016.Andersson K (2018) This Month in Investigative UrologyJournal of Urology, VOL. 192, NO. 2, (295-296), Online publication date: 1-Aug-2014.Street J, Yuen P and Star R (2018) Bioactive Exosomes: Possibilities for Diagnosis and Management of Bladder CancerJournal of Urology, VOL. 192, NO. 2, (297-298), Online publication date: 1-Aug-2014. Volume 192Issue 2August 2014Page: 583-592Supplementary Materials Advertisement Copyright & Permissions© 2014 by American Urological Association Education and Research, Inc.Keywordsurinary bladderhumandisease progressionexosomesEDIL-3 proteinurinary bladder neoplasmsAcknowledgmentsDr. Reznikoff provided SV-HUC cells. The Berk Laboratory, University of Rochester provided HUVECs. C.R. Silvers provided technical assistance. M. Sowden and S. Majumder, CVRI, provided HUVEC cells and protocols. Jonathan Flax provided insight. Roy R. Parker read the manuscript and provided editorial advice. L. Maquat read the manuscript and provided insight into experiments. H. Miyamoto made pathological diagnoses and read slides. K. Bentley, Electron Microscopy Research Core, provided assistance. B. Smith and S. Schoen assisted with institutional review board materials. M. Kiernan enrolled patients.MetricsAuthor Information Carla J. Beckham Department of Urology, University of Rochester, Rochester, New York More articles by this author Jayme Olsen Department of Genetics, University of Rochester, Rochester, New York More articles by this author Peng-Nien Yin Department of Urology, University of Rochester, Rochester, New York More articles by this author Chia-Hao Wu Department of Urology, University of Rochester, Rochester, New York More articles by this author Huei-Ju Ting Department of Biological Science and Technology, National University of Tainan, Taiwan, Republic of China More articles by this author Fred K. Hagen Department of Biochemistry and Biophysics Proteomics Center, University of Rochester, Rochester, New York More articles by this author Emelian Scosyrev Department of Urology, University of Rochester, Rochester, New York More articles by this author Edward M. Messing Department of Urology, University of Rochester, Rochester, New York More articles by this author Yi-Fen Lee Department of Urology, University of Rochester, Rochester, New York More articles by this author Expand All Advertisement PDF downloadLoading ..." @default.
• W2024883517 created "2016-06-24" @default.
• W2024883517 creator A5006971751 @default.
• W2024883517 creator A5009708128 @default.
• W2024883517 creator A5014785898 @default.
• W2024883517 creator A5018785108 @default.
• W2024883517 creator A5051018110 @default.
• W2024883517 creator A5079161431 @default.
• W2024883517 creator A5083067973 @default.
• W2024883517 creator A5090720446 @default.
• W2024883517 creator A5090812771 @default.
• W2024883517 date "2014-08-01" @default.
• W2024883517 modified "2023-10-11" @default.
• W2024883517 title "Bladder Cancer Exosomes Contain EDIL-3/Del1 and Facilitate Cancer Progression" @default.
• W2024883517 cites W1762620449 @default.
• W2024883517 cites W1786980 @default.
• W2024883517 cites W1877686344 @default.
• W2024883517 cites W1965774359 @default.
• W2024883517 cites W1975859224 @default.
• W2024883517 cites W1976721329 @default.
• W2024883517 cites W1984933192 @default.
• W2024883517 cites W1991390602 @default.
• W2024883517 cites W1999065310 @default.
• W2024883517 cites W2006680819 @default.
• W2024883517 cites W2007226924 @default.
• W2024883517 cites W2015186671 @default.
• W2024883517 cites W2019917125 @default.
• W2024883517 cites W2020245457 @default.
• W2024883517 cites W2030701238 @default.
• W2024883517 cites W2046571883 @default.
• W2024883517 cites W2050935473 @default.
• W2024883517 cites W2052706090 @default.
• W2024883517 cites W2055313665 @default.
• W2024883517 cites W2063480251 @default.
• W2024883517 cites W2066522455 @default.
• W2024883517 cites W2084800734 @default.
• W2024883517 cites W2097343453 @default.
• W2024883517 cites W2100592123 @default.
• W2024883517 cites W2102250130 @default.
• W2024883517 cites W2111778115 @default.
• W2024883517 cites W2169491358 @default.
• W2024883517 cites W2417929113 @default.
• W2024883517 doi "https://doi.org/10.1016/j.juro.2014.02.035" @default.
• W2024883517 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/24530986" @default.
• W2024883517 hasPublicationYear "2014" @default.
• W2024883517 type Work @default.
• W2024883517 sameAs 2024883517 @default.
• W2024883517 citedByCount "151" @default.
• W2024883517 countsByYear W20248835172014 @default.
• W2024883517 countsByYear W20248835172015 @default.
• W2024883517 countsByYear W20248835172016 @default.
• W2024883517 countsByYear W20248835172017 @default.
• W2024883517 countsByYear W20248835172018 @default.
• W2024883517 countsByYear W20248835172019 @default.
• W2024883517 countsByYear W20248835172020 @default.
• W2024883517 countsByYear W20248835172021 @default.
• W2024883517 countsByYear W20248835172022 @default.
• W2024883517 countsByYear W20248835172023 @default.
• W2024883517 crossrefType "journal-article" @default.
• W2024883517 hasAuthorship W2024883517A5006971751 @default.
• W2024883517 hasAuthorship W2024883517A5009708128 @default.
• W2024883517 hasAuthorship W2024883517A5014785898 @default.
• W2024883517 hasAuthorship W2024883517A5018785108 @default.
• W2024883517 hasAuthorship W2024883517A5051018110 @default.
• W2024883517 hasAuthorship W2024883517A5079161431 @default.
• W2024883517 hasAuthorship W2024883517A5083067973 @default.
• W2024883517 hasAuthorship W2024883517A5090720446 @default.
• W2024883517 hasAuthorship W2024883517A5090812771 @default.
• W2024883517 hasConcept C104317684 @default.
• W2024883517 hasConcept C121608353 @default.
• W2024883517 hasConcept C126322002 @default.
• W2024883517 hasConcept C143998085 @default.
• W2024883517 hasConcept C145059251 @default.
• W2024883517 hasConcept C20518536 @default.
• W2024883517 hasConcept C2780352672 @default.
• W2024883517 hasConcept C502942594 @default.
• W2024883517 hasConcept C54355233 @default.
• W2024883517 hasConcept C71924100 @default.
• W2024883517 hasConcept C86803240 @default.
• W2024883517 hasConceptScore W2024883517C104317684 @default.
• W2024883517 hasConceptScore W2024883517C121608353 @default.
• W2024883517 hasConceptScore W2024883517C126322002 @default.
• W2024883517 hasConceptScore W2024883517C143998085 @default.
• W2024883517 hasConceptScore W2024883517C145059251 @default.
• W2024883517 hasConceptScore W2024883517C20518536 @default.
• W2024883517 hasConceptScore W2024883517C2780352672 @default.
• W2024883517 hasConceptScore W2024883517C502942594 @default.
• W2024883517 hasConceptScore W2024883517C54355233 @default.
• W2024883517 hasConceptScore W2024883517C71924100 @default.
• W2024883517 hasConceptScore W2024883517C86803240 @default.
• W2024883517 hasIssue "2" @default.
• W2024883517 hasLocation W20248835171 @default.
• W2024883517 hasLocation W20248835172 @default.
• W2024883517 hasOpenAccess W2024883517 @default.
• W2024883517 hasPrimaryLocation W20248835171 @default.
• W2024883517 hasRelatedWork W1979321249 @default.
• W2024883517 hasRelatedWork W2011500441 @default.
• W2024883517 hasRelatedWork W2144610094 @default.

• next