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• W2024892252 abstract "To develop a real-time Taq nuclease assay (TNA) to enable the in vivo enumeration of Megasphaera elsdenii.Megasphaera elsdenii YE34 was phenotypically characteristic of the species and had 16S rDNA sequence similarity of 98% to previously described isolates. Calibration of the number of cells of M. elsdenii against the cycle threshold of fluorescent dye release gave a straight-line relationship with a correlation coefficient approximating unity. The specificity of the assay for M. elsdenii was confirmed by performing it against a panel of 24 heterogeneous, mainly ruminal bacteria. Megasphaera elsdenii was not detected in ruminal contents from a pasture-fed steer but was readily detected 2 and 50 h after the probiotic introduction of the bacterium into the rumen.Real-time TNA has provided a sensitive and specific means of enumerating the M. elsdenii population in rumen contents.Megasphaera elsdenii is an important lactate-degrading ruminal bacterium that has been selected for probiotic use to prevent acidosis and enhance starch utilization in grain-fed cattle. The assay developed in this study provides a tool for determining the ability of probiotically-introduced M. elsdenii to establish useful populations in the rumen." @default.
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• W2024892252 date "2002-04-01" @default.
• W2024892252 modified "2023-10-03" @default.
• W2024892252 title "Enumeration of Megasphaera elsdenii in rumen contents by real-time Taq nuclease assay" @default.
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• W2024892252 doi "https://doi.org/10.1046/j.1365-2672.2002.01580.x" @default.
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