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- W2024893175 abstract "A rapid procedure for the purification of RNA polymerase II from Saccharomyces cerevisiae is described. Total RNA polymerase activity was solubilized from whole cells by sonication in 0.32 M (NH4)2SO4 and RNA polymerase II purified by polyethylenimine fractionation, ammonium sulfate precipitation, and chromatography on DEAE-cellulose, DEAE-Sephadex, and phosphocellulose. The procedure may be completed in 2.5 days and the resultant enzyme is judged to be greater than 90% pure." @default.
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- W2024893175 date "1983-02-01" @default.
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- W2024893175 title "Purification of DNA-dependent RNA polymerase II from Saccharomyces cerevisiae" @default.
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- W2024893175 doi "https://doi.org/10.1016/0003-2697(83)90378-0" @default.
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