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- W2024934626 abstract "An assay method in a reversed micellar system, which can be better characterized in physicochemical terms than that in an emulsion system, was investigated. The first-order rate constant when [S] « Km and the zero-order rate constant when [S]» Km for lipase reaction in the system show a linear relationship with enzyme concentration over a twenty-fold range, which is an important criterion for an enzyme assay. Based on the results, a three-point assay of lipase in reversed micelles was established. To ensure the initial rate, the assay time should be limited to the time during which about 4.5% of the substrate is utilized." @default.
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- W2024934626 date "1987-01-01" @default.
- W2024934626 modified "2023-10-18" @default.
- W2024934626 title "Determination of lipase activity in AOT-isooctane reversed micelles." @default.
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- W2024934626 doi "https://doi.org/10.1271/bbb1961.51.615" @default.
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