Matches in SemOpenAlex for { <https://semopenalex.org/work/W2024946711> ?p ?o ?g. }
Showing items 1 to 71 of
71
with 100 items per page.
- W2024946711 endingPage "2440" @default.
- W2024946711 startingPage "2435" @default.
- W2024946711 abstract "αsl-Casein can be made either soluble or insoluble by adjusting the concentration of coexisting calcium ions. In this study, we tried to make a soluble-insoluble interconvertible enzyme through the formation of a conjugate of an enzyme and αsl-casein using a heterobifunctional crosslinking reagent, N-succinimidyl 3-(2-pyridyldithio)propionate. The conjugate of phosphoglyceromutase and native αs1-casein did not exhibit sufficient calcium-dependent precipitation. However, conjugates of enzymes (phosphoglyceromutase, enolase or peroxidase) and αsl-casein polymerized by transglutaminase precipitated almost completely in the presence of more than 50 mM CaCl2. Most of the enzyme conjugates precipitated as calcium caseinates could be solubilized reversibly with EDTA, without a significant loss of activity. A mixture of the enzyme • polymerized αs1-casein conjugates prepared with phosphoglyceromutase, enolase and pyruvate kinase could catalyze sequential reactions which convert d-3-phosphoglycerate into pyruvate with the same efficiency as a mixture of free enzymes. These results indicate that conjugates of enzymes and polymerized αs1-casein can be useful as soluble-insoluble interconvertible enzymes." @default.
- W2024946711 created "2016-06-24" @default.
- W2024946711 creator A5001644765 @default.
- W2024946711 creator A5045895101 @default.
- W2024946711 creator A5075464329 @default.
- W2024946711 creator A5079098808 @default.
- W2024946711 creator A5086963618 @default.
- W2024946711 date "1984-10-01" @default.
- W2024946711 modified "2023-09-27" @default.
- W2024946711 title "Preparation of Soluble-insoluble Interconvertible Enzymes: Enzyme • Polymerized αsl-Casein Conjugates" @default.
- W2024946711 cites W4247429897 @default.
- W2024946711 cites W7609898 @default.
- W2024946711 cites W958087039 @default.
- W2024946711 doi "https://doi.org/10.1080/00021369.1984.10866527" @default.
- W2024946711 hasPublicationYear "1984" @default.
- W2024946711 type Work @default.
- W2024946711 sameAs 2024946711 @default.
- W2024946711 citedByCount "1" @default.
- W2024946711 crossrefType "journal-article" @default.
- W2024946711 hasAuthorship W2024946711A5001644765 @default.
- W2024946711 hasAuthorship W2024946711A5045895101 @default.
- W2024946711 hasAuthorship W2024946711A5075464329 @default.
- W2024946711 hasAuthorship W2024946711A5079098808 @default.
- W2024946711 hasAuthorship W2024946711A5086963618 @default.
- W2024946711 hasConcept C134306372 @default.
- W2024946711 hasConcept C178790620 @default.
- W2024946711 hasConcept C181199279 @default.
- W2024946711 hasConcept C185592680 @default.
- W2024946711 hasConcept C197336794 @default.
- W2024946711 hasConcept C2776142715 @default.
- W2024946711 hasConcept C33923547 @default.
- W2024946711 hasConcept C40875361 @default.
- W2024946711 hasConcept C43617362 @default.
- W2024946711 hasConcept C44228677 @default.
- W2024946711 hasConcept C519063684 @default.
- W2024946711 hasConcept C521977710 @default.
- W2024946711 hasConcept C55493867 @default.
- W2024946711 hasConceptScore W2024946711C134306372 @default.
- W2024946711 hasConceptScore W2024946711C178790620 @default.
- W2024946711 hasConceptScore W2024946711C181199279 @default.
- W2024946711 hasConceptScore W2024946711C185592680 @default.
- W2024946711 hasConceptScore W2024946711C197336794 @default.
- W2024946711 hasConceptScore W2024946711C2776142715 @default.
- W2024946711 hasConceptScore W2024946711C33923547 @default.
- W2024946711 hasConceptScore W2024946711C40875361 @default.
- W2024946711 hasConceptScore W2024946711C43617362 @default.
- W2024946711 hasConceptScore W2024946711C44228677 @default.
- W2024946711 hasConceptScore W2024946711C519063684 @default.
- W2024946711 hasConceptScore W2024946711C521977710 @default.
- W2024946711 hasConceptScore W2024946711C55493867 @default.
- W2024946711 hasIssue "10" @default.
- W2024946711 hasLocation W20249467111 @default.
- W2024946711 hasOpenAccess W2024946711 @default.
- W2024946711 hasPrimaryLocation W20249467111 @default.
- W2024946711 hasRelatedWork W2024946711 @default.
- W2024946711 hasRelatedWork W2233568395 @default.
- W2024946711 hasRelatedWork W2949098585 @default.
- W2024946711 hasRelatedWork W2949523070 @default.
- W2024946711 hasRelatedWork W2950902791 @default.
- W2024946711 hasRelatedWork W2952256733 @default.
- W2024946711 hasRelatedWork W2952360817 @default.
- W2024946711 hasRelatedWork W2952417160 @default.
- W2024946711 hasRelatedWork W2952556931 @default.
- W2024946711 hasRelatedWork W4240338621 @default.
- W2024946711 hasVolume "48" @default.
- W2024946711 isParatext "false" @default.
- W2024946711 isRetracted "false" @default.
- W2024946711 magId "2024946711" @default.
- W2024946711 workType "article" @default.