Matches in SemOpenAlex for { <https://semopenalex.org/work/W2024992445> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W2024992445 endingPage "9736" @default.
- W2024992445 startingPage "9728" @default.
- W2024992445 abstract "Recognition-based assembly of micron- to nano-sized colloidal particles functionalized with DNA has generated great interest in the past decade; however, reversing the assembly process is typically achieved by thermal denaturation of the oligonucleotide duplexes. Here, we report an alternative disassembly approach at a fixed temperature using competitive hybridization events between immobilized and soluble oligonucleotide strands. Microspheres are first aggregated via primary hybridization events between immobilized DNA strands with a weak, but sufficient, affinity for partner strands to link complementary surfaces together. To reverse the aggregation process, soluble oligonucleotides are then added to competitively displace the original hybridization partners through secondary hybridization events. Using flow cytometry to quantify hybridization events and microscopy to examine DNA-mediated aggregation and redispersion, we found that the efficiency of competitive displacement is based upon (1) the difference in base pair matches between the primary and secondary target for the same probe sequence and (2) the concentration of hybridizing oligonucleotides participating in microsphere aggregation. To the best of our knowledge, this study is the first to employ DNA hybridization events to mediate reversible adhesion between colloidal particles at a fixed temperature." @default.
- W2024992445 created "2016-06-24" @default.
- W2024992445 creator A5043488897 @default.
- W2024992445 creator A5081458195 @default.
- W2024992445 date "2007-08-15" @default.
- W2024992445 modified "2023-09-29" @default.
- W2024992445 title "Reversing DNA-Mediated Adhesion at a Fixed Temperature" @default.
- W2024992445 cites W1972228705 @default.
- W2024992445 cites W2013141063 @default.
- W2024992445 cites W2015786305 @default.
- W2024992445 cites W2033239668 @default.
- W2024992445 cites W2037015381 @default.
- W2024992445 cites W2044507323 @default.
- W2024992445 cites W2052566608 @default.
- W2024992445 cites W2056785880 @default.
- W2024992445 cites W2080955003 @default.
- W2024992445 cites W2085568007 @default.
- W2024992445 cites W2091131890 @default.
- W2024992445 cites W2123314710 @default.
- W2024992445 cites W2130243293 @default.
- W2024992445 cites W2135995702 @default.
- W2024992445 cites W2137475948 @default.
- W2024992445 cites W2141157874 @default.
- W2024992445 cites W2149681483 @default.
- W2024992445 cites W2160763121 @default.
- W2024992445 cites W2323809606 @default.
- W2024992445 cites W4235092688 @default.
- W2024992445 doi "https://doi.org/10.1021/la700601j" @default.
- W2024992445 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/17696456" @default.
- W2024992445 hasPublicationYear "2007" @default.
- W2024992445 type Work @default.
- W2024992445 sameAs 2024992445 @default.
- W2024992445 citedByCount "45" @default.
- W2024992445 countsByYear W20249924452012 @default.
- W2024992445 countsByYear W20249924452013 @default.
- W2024992445 countsByYear W20249924452014 @default.
- W2024992445 countsByYear W20249924452015 @default.
- W2024992445 countsByYear W20249924452016 @default.
- W2024992445 countsByYear W20249924452017 @default.
- W2024992445 countsByYear W20249924452018 @default.
- W2024992445 countsByYear W20249924452020 @default.
- W2024992445 countsByYear W20249924452022 @default.
- W2024992445 countsByYear W20249924452023 @default.
- W2024992445 crossrefType "journal-article" @default.
- W2024992445 hasAuthorship W2024992445A5043488897 @default.
- W2024992445 hasAuthorship W2024992445A5081458195 @default.
- W2024992445 hasConcept C104317684 @default.
- W2024992445 hasConcept C12554922 @default.
- W2024992445 hasConcept C129312508 @default.
- W2024992445 hasConcept C137390916 @default.
- W2024992445 hasConcept C150194340 @default.
- W2024992445 hasConcept C171250308 @default.
- W2024992445 hasConcept C178790620 @default.
- W2024992445 hasConcept C185592680 @default.
- W2024992445 hasConcept C192562407 @default.
- W2024992445 hasConcept C3017666073 @default.
- W2024992445 hasConcept C54871578 @default.
- W2024992445 hasConcept C552990157 @default.
- W2024992445 hasConcept C55493867 @default.
- W2024992445 hasConcept C81439078 @default.
- W2024992445 hasConcept C84416704 @default.
- W2024992445 hasConcept C86803240 @default.
- W2024992445 hasConceptScore W2024992445C104317684 @default.
- W2024992445 hasConceptScore W2024992445C12554922 @default.
- W2024992445 hasConceptScore W2024992445C129312508 @default.
- W2024992445 hasConceptScore W2024992445C137390916 @default.
- W2024992445 hasConceptScore W2024992445C150194340 @default.
- W2024992445 hasConceptScore W2024992445C171250308 @default.
- W2024992445 hasConceptScore W2024992445C178790620 @default.
- W2024992445 hasConceptScore W2024992445C185592680 @default.
- W2024992445 hasConceptScore W2024992445C192562407 @default.
- W2024992445 hasConceptScore W2024992445C3017666073 @default.
- W2024992445 hasConceptScore W2024992445C54871578 @default.
- W2024992445 hasConceptScore W2024992445C552990157 @default.
- W2024992445 hasConceptScore W2024992445C55493867 @default.
- W2024992445 hasConceptScore W2024992445C81439078 @default.
- W2024992445 hasConceptScore W2024992445C84416704 @default.
- W2024992445 hasConceptScore W2024992445C86803240 @default.
- W2024992445 hasIssue "19" @default.
- W2024992445 hasLocation W20249924451 @default.
- W2024992445 hasLocation W20249924452 @default.
- W2024992445 hasOpenAccess W2024992445 @default.
- W2024992445 hasPrimaryLocation W20249924451 @default.
- W2024992445 hasRelatedWork W1972864282 @default.
- W2024992445 hasRelatedWork W1988244303 @default.
- W2024992445 hasRelatedWork W2041979346 @default.
- W2024992445 hasRelatedWork W2048291142 @default.
- W2024992445 hasRelatedWork W2066656078 @default.
- W2024992445 hasRelatedWork W2110847890 @default.
- W2024992445 hasRelatedWork W2133608598 @default.
- W2024992445 hasRelatedWork W2151200650 @default.
- W2024992445 hasRelatedWork W2289686375 @default.
- W2024992445 hasRelatedWork W2186433638 @default.
- W2024992445 hasVolume "23" @default.
- W2024992445 isParatext "false" @default.
- W2024992445 isRetracted "false" @default.
- W2024992445 magId "2024992445" @default.
- W2024992445 workType "article" @default.