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- W2024992795 abstract "Abstract Over the years, conventional Stahl‐type thin‐layer chromatography (TLC) chambers (i.e., N‐chambers) have attracted criticism for more than one reason. For example, practitioners have questioned the value of the time‐consuming procedure of saturating these chambers with mobile‐phase vapor, and those who focus on the theory of planar chromatography, have complained that the well‐known stepwise diminishing of the rate of capillary flow of the mobile phase in the ascending mode makes computational optimization of solute retention more difficult and less accurate than it could be at constant flow. Everybody also readily agrees that mobile phase consumption is much higher in N‐chambers than in sandwich chambers (S‐chambers). The economic superiority of S‐chambers cannot, however, be regarded as a decisive factor when it comes to making a choice of the TLC equipment, because physicochemical aspects of the chromatographic process are more important. In the work discussed in this paper, we compared the N‐type and S‐type chambers in terms of mobile phase flow rate, the R F values obtained, and the densitometrically established concentration profiles of the test analytes. For this comparison, we selected several phenyl‐substituted alcohols and acids, low‐activity adsorbents (cellulose powder and chromatographic paper), and low‐polarity solvents (n‐octane and decalin) as the components of the chromatographic systems investigated. The overall conclusion from the results obtained is that the physicochemical performance of the S‐chamber is comparable with that of the N‐chamber, and the only indisputable superiority of the S‐chamber is the more economical use of the mobile phases." @default.
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- W2024992795 date "2004-01-01" @default.
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- W2024992795 title "Densitometric Comparison of the Performance of Stahl‐Type and Sandwich‐Type Planar Chromatographic Chambers" @default.
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- W2024992795 doi "https://doi.org/10.1081/jlc-120039414" @default.
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