Matches in SemOpenAlex for { <https://semopenalex.org/work/W2024992947> ?p ?o ?g. }
- W2024992947 endingPage "290" @default.
- W2024992947 startingPage "279" @default.
- W2024992947 abstract "The Schistosoma mansoni glutathione-S-transferase with an apparent molecular mass of 28 kDa, Smp28, has a blocked N-terminus which has been elucidated with the aid of the cDNA sequence combined with mass spectrometry and amino acid composition analysis of the N-terminal tryptic peptide. The blocked N-terminal tryptic peptide (m/z 695.8) contained an equimolar ratio of E, G, H, A, I and K3 upon amino acid composition analysis in agreement with its expected sequence AGEHIK, and showed a delta m = +41.7 Da compared to the predicted mass, which is consistent with the N-terminal alanine being acetylated (delta m = +42.0 Da). The mass of the complete molecule (23,744.5 +/- 3.3 Da) determined by electrospray mass spectrometry showed a further mass increase of 14 Da with respect to Smp28 containing an N-acetylated alanine. This result is consistent with one of the seven methionines being present as a methionine sulfoxide in ca. 90% of the Smp28 molecules in this preparation. Tryptic mapping of Smp28 showed five of the seven methionines to be partially oxidized by mass spectrometry. This is indicative of the ease with which this modification occurs. Two minor components were detected along with the intact molecule, corresponding to modified forms of the molecule, originating from reaction of the only cysteine residue either with itself forming a covalent dimer or with glutathione. On-line liquid chromatography-mass spectrometry has been compared with the off-line complete tryptic map of Smp28 confirming 97% of the primary structure in less than 2 h." @default.
- W2024992947 created "2016-06-24" @default.
- W2024992947 creator A5006922451 @default.
- W2024992947 creator A5010920048 @default.
- W2024992947 creator A5024394171 @default.
- W2024992947 creator A5031681383 @default.
- W2024992947 creator A5042491473 @default.
- W2024992947 creator A5058316837 @default.
- W2024992947 creator A5067240461 @default.
- W2024992947 creator A5090792036 @default.
- W2024992947 date "1994-12-01" @default.
- W2024992947 modified "2023-09-27" @default.
- W2024992947 title "Analysis of the primary structure and post-translational modifications of the Schistosoma mansoni antigen Smp28 by electrospray mass spectrometry" @default.
- W2024992947 cites W147697679 @default.
- W2024992947 cites W1536916609 @default.
- W2024992947 cites W1545896381 @default.
- W2024992947 cites W1555049966 @default.
- W2024992947 cites W1561939009 @default.
- W2024992947 cites W1580890039 @default.
- W2024992947 cites W1911906532 @default.
- W2024992947 cites W1969018508 @default.
- W2024992947 cites W1969419384 @default.
- W2024992947 cites W1988287443 @default.
- W2024992947 cites W1997657115 @default.
- W2024992947 cites W2006985642 @default.
- W2024992947 cites W2013065176 @default.
- W2024992947 cites W2046788097 @default.
- W2024992947 cites W2057183687 @default.
- W2024992947 cites W2068790245 @default.
- W2024992947 cites W2085015489 @default.
- W2024992947 cites W2092846883 @default.
- W2024992947 cites W2092997121 @default.
- W2024992947 cites W2100837269 @default.
- W2024992947 cites W2123148055 @default.
- W2024992947 cites W2170436706 @default.
- W2024992947 cites W2177928811 @default.
- W2024992947 cites W2282243275 @default.
- W2024992947 cites W2417515531 @default.
- W2024992947 cites W251629702 @default.
- W2024992947 doi "https://doi.org/10.1016/0378-4347(94)00298-3" @default.
- W2024992947 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7719482" @default.
- W2024992947 hasPublicationYear "1994" @default.
- W2024992947 type Work @default.
- W2024992947 sameAs 2024992947 @default.
- W2024992947 citedByCount "12" @default.
- W2024992947 countsByYear W20249929472021 @default.
- W2024992947 crossrefType "journal-article" @default.
- W2024992947 hasAuthorship W2024992947A5006922451 @default.
- W2024992947 hasAuthorship W2024992947A5010920048 @default.
- W2024992947 hasAuthorship W2024992947A5024394171 @default.
- W2024992947 hasAuthorship W2024992947A5031681383 @default.
- W2024992947 hasAuthorship W2024992947A5042491473 @default.
- W2024992947 hasAuthorship W2024992947A5058316837 @default.
- W2024992947 hasAuthorship W2024992947A5067240461 @default.
- W2024992947 hasAuthorship W2024992947A5090792036 @default.
- W2024992947 hasConcept C104317684 @default.
- W2024992947 hasConcept C162356407 @default.
- W2024992947 hasConcept C167050937 @default.
- W2024992947 hasConcept C167625842 @default.
- W2024992947 hasConcept C181199279 @default.
- W2024992947 hasConcept C185592680 @default.
- W2024992947 hasConcept C202157981 @default.
- W2024992947 hasConcept C2779281246 @default.
- W2024992947 hasConcept C40684141 @default.
- W2024992947 hasConcept C43617362 @default.
- W2024992947 hasConcept C55493867 @default.
- W2024992947 hasConcept C58121356 @default.
- W2024992947 hasConcept C92825506 @default.
- W2024992947 hasConceptScore W2024992947C104317684 @default.
- W2024992947 hasConceptScore W2024992947C162356407 @default.
- W2024992947 hasConceptScore W2024992947C167050937 @default.
- W2024992947 hasConceptScore W2024992947C167625842 @default.
- W2024992947 hasConceptScore W2024992947C181199279 @default.
- W2024992947 hasConceptScore W2024992947C185592680 @default.
- W2024992947 hasConceptScore W2024992947C202157981 @default.
- W2024992947 hasConceptScore W2024992947C2779281246 @default.
- W2024992947 hasConceptScore W2024992947C40684141 @default.
- W2024992947 hasConceptScore W2024992947C43617362 @default.
- W2024992947 hasConceptScore W2024992947C55493867 @default.
- W2024992947 hasConceptScore W2024992947C58121356 @default.
- W2024992947 hasConceptScore W2024992947C92825506 @default.
- W2024992947 hasIssue "2" @default.
- W2024992947 hasLocation W20249929471 @default.
- W2024992947 hasLocation W20249929472 @default.
- W2024992947 hasOpenAccess W2024992947 @default.
- W2024992947 hasPrimaryLocation W20249929471 @default.
- W2024992947 hasRelatedWork W1560616354 @default.
- W2024992947 hasRelatedWork W1982827588 @default.
- W2024992947 hasRelatedWork W1999223590 @default.
- W2024992947 hasRelatedWork W2022466283 @default.
- W2024992947 hasRelatedWork W2038649657 @default.
- W2024992947 hasRelatedWork W2040286337 @default.
- W2024992947 hasRelatedWork W2052460101 @default.
- W2024992947 hasRelatedWork W2082854124 @default.
- W2024992947 hasRelatedWork W2365251712 @default.
- W2024992947 hasRelatedWork W277754798 @default.
- W2024992947 hasVolume "662" @default.
- W2024992947 isParatext "false" @default.