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• W2024998610 abstract "In this paper, we describe a method for size-based electrophoretic separation of sodium dodecyl sulfate (SDS)–protein complexes on a polymethyl methacrylate (PMMA) microchip, using a separation buffer solution containing SDS and linear polyacrylamide as a sieving matrix. We developed optimum conditions under which protein separations can be performed, using polyethylene glycol (PEG)-coated polymer microchips and electrokinetic sample injection. We studied the performance of protein separations on the PEG-coated PMMA microchip. The electrophoretic separation of proteins (21.5–116.0 kDa) was completed with separation lengths of 3 mm, achieved within 8 s on the PEG-coated microchip. This high-speed method may be applied to protein separations over a large range of molecular weight, making the PEG-coated microchip approach applicable to high-speed proteome analysis systems." @default.
• W2024998610 created "2016-06-24" @default.
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• W2024998610 date "2005-07-01" @default.
• W2024998610 modified "2023-10-17" @default.
• W2024998610 title "High-speed separation of proteins by microchip electrophoresis using a polyethylene glycol-coated plastic chip with a sodium dodecyl sulfate-linear polyacrylamide solution" @default.
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• W2024998610 doi "https://doi.org/10.1002/elps.200410337" @default.
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