Matches in SemOpenAlex for { <https://semopenalex.org/work/W2025114711> ?p ?o ?g. }
- W2025114711 endingPage "91" @default.
- W2025114711 startingPage "78" @default.
- W2025114711 abstract "Cathepsin K is the major collagenolytic enzyme produced by bone-resorbing osteoclasts. We showed earlier that the unique triple-helical collagen-degrading activity of cathepsin K depends on the formation of complexes with bone-or cartilage-resident glycosaminoglycans, such as chondroitin 4-sulfate (C4-S). Here, we describe the crystal structure of a 1:n complex of cathepsin K:C4-S inhibited by E64 at a resolution of 1.8 Å. The overall structure reveals an unusual “beads-on-a-string”-like organization. Multiple cathepsin K molecules bind specifically to a single cosine curve-shaped strand of C4-S with each cathepsin K molecule interacting with three disaccharide residues of C4-S. One of the more important sets of interactions comes from a single turn of helix close to the N terminus of the proteinase containing a basic amino acid triplet (Arg8-Lys9-Lys10) that forms multiple hydrogen bonds either to the caboxylate or to the 4-sulfate groups of C4-S. Altogether, the binding sites with C4-S are located in the R-domain of cathepsin K and are distant from its active site. This explains why the general proteolytic activity of cathepsin K is not affected by the binding of chondroitin sulfate. Biochemical analyses of cathepsin K and C4-S mixtures support the presence of a 1:n complex in solution; a dissociation constant, Kd, of about 10 nM was determined for the interaction between cathepsin K and C4-S." @default.
- W2025114711 created "2016-06-24" @default.
- W2025114711 creator A5008405644 @default.
- W2025114711 creator A5035792194 @default.
- W2025114711 creator A5036927633 @default.
- W2025114711 creator A5062362734 @default.
- W2025114711 creator A5066390455 @default.
- W2025114711 date "2008-10-01" @default.
- W2025114711 modified "2023-10-17" @default.
- W2025114711 title "The Crystal and Molecular Structures of a Cathepsin K:Chondroitin Sulfate Complex" @default.
- W2025114711 cites W1594498062 @default.
- W2025114711 cites W1762318613 @default.
- W2025114711 cites W1812339273 @default.
- W2025114711 cites W1965144118 @default.
- W2025114711 cites W1968024679 @default.
- W2025114711 cites W1989560280 @default.
- W2025114711 cites W1991947705 @default.
- W2025114711 cites W1993404146 @default.
- W2025114711 cites W1995017064 @default.
- W2025114711 cites W2010127700 @default.
- W2025114711 cites W2014694459 @default.
- W2025114711 cites W2017614987 @default.
- W2025114711 cites W2021453375 @default.
- W2025114711 cites W2043914705 @default.
- W2025114711 cites W2049891971 @default.
- W2025114711 cites W2058616210 @default.
- W2025114711 cites W2067439258 @default.
- W2025114711 cites W2075775518 @default.
- W2025114711 cites W2078146467 @default.
- W2025114711 cites W2086201428 @default.
- W2025114711 cites W2090299301 @default.
- W2025114711 cites W2090348431 @default.
- W2025114711 cites W2092751827 @default.
- W2025114711 cites W2096464236 @default.
- W2025114711 cites W2096716204 @default.
- W2025114711 cites W2102008951 @default.
- W2025114711 cites W2109350686 @default.
- W2025114711 cites W2110893691 @default.
- W2025114711 cites W2120565090 @default.
- W2025114711 cites W2135839939 @default.
- W2025114711 cites W2161115679 @default.
- W2025114711 cites W2333911447 @default.
- W2025114711 cites W4210675630 @default.
- W2025114711 doi "https://doi.org/10.1016/j.jmb.2008.07.038" @default.
- W2025114711 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/18692071" @default.
- W2025114711 hasPublicationYear "2008" @default.
- W2025114711 type Work @default.
- W2025114711 sameAs 2025114711 @default.
- W2025114711 citedByCount "87" @default.
- W2025114711 countsByYear W20251147112012 @default.
- W2025114711 countsByYear W20251147112013 @default.
- W2025114711 countsByYear W20251147112014 @default.
- W2025114711 countsByYear W20251147112015 @default.
- W2025114711 countsByYear W20251147112016 @default.
- W2025114711 countsByYear W20251147112017 @default.
- W2025114711 countsByYear W20251147112018 @default.
- W2025114711 countsByYear W20251147112019 @default.
- W2025114711 countsByYear W20251147112020 @default.
- W2025114711 countsByYear W20251147112021 @default.
- W2025114711 countsByYear W20251147112022 @default.
- W2025114711 countsByYear W20251147112023 @default.
- W2025114711 crossrefType "journal-article" @default.
- W2025114711 hasAuthorship W2025114711A5008405644 @default.
- W2025114711 hasAuthorship W2025114711A5035792194 @default.
- W2025114711 hasAuthorship W2025114711A5036927633 @default.
- W2025114711 hasAuthorship W2025114711A5062362734 @default.
- W2025114711 hasAuthorship W2025114711A5066390455 @default.
- W2025114711 hasConcept C101990758 @default.
- W2025114711 hasConcept C153074725 @default.
- W2025114711 hasConcept C167844969 @default.
- W2025114711 hasConcept C170493617 @default.
- W2025114711 hasConcept C181199279 @default.
- W2025114711 hasConcept C185592680 @default.
- W2025114711 hasConcept C2776033226 @default.
- W2025114711 hasConcept C2776735749 @default.
- W2025114711 hasConcept C2779553658 @default.
- W2025114711 hasConcept C2780034444 @default.
- W2025114711 hasConcept C28021979 @default.
- W2025114711 hasConcept C51618164 @default.
- W2025114711 hasConcept C55493867 @default.
- W2025114711 hasConcept C71240020 @default.
- W2025114711 hasConceptScore W2025114711C101990758 @default.
- W2025114711 hasConceptScore W2025114711C153074725 @default.
- W2025114711 hasConceptScore W2025114711C167844969 @default.
- W2025114711 hasConceptScore W2025114711C170493617 @default.
- W2025114711 hasConceptScore W2025114711C181199279 @default.
- W2025114711 hasConceptScore W2025114711C185592680 @default.
- W2025114711 hasConceptScore W2025114711C2776033226 @default.
- W2025114711 hasConceptScore W2025114711C2776735749 @default.
- W2025114711 hasConceptScore W2025114711C2779553658 @default.
- W2025114711 hasConceptScore W2025114711C2780034444 @default.
- W2025114711 hasConceptScore W2025114711C28021979 @default.
- W2025114711 hasConceptScore W2025114711C51618164 @default.
- W2025114711 hasConceptScore W2025114711C55493867 @default.
- W2025114711 hasConceptScore W2025114711C71240020 @default.
- W2025114711 hasIssue "1" @default.
- W2025114711 hasLocation W20251147111 @default.
- W2025114711 hasLocation W20251147112 @default.