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- W2025116989 abstract "Purification of target oligodeoxyribonucleotides from failure sequence by-products of synthesis is often required for polymerase chain reaction primers, DNA sequencing and other oligonucleotide applications. We have developed purification protocols based on a reversed-phase mechanism (“trityl on” purification) using a 96-well Oasis HLB extraction plate. The Oasis HLB sorbent combines excellent pH stability with a high loading capacity allowing for single-step purification of 0.2 μM scale synthesis. After sample loading and washing, the oligonucleotide trityl group is cleaved on the plate with 2% trifluoroacetic acid. Target DNA is eluted with acetonitrile–0.36 mM triethylamine acetate, pH 11.3 (10:90, v/v). Typical yield of purified product is 60–95%. Final purity, measured by capillary gel electrophoresis, was found to be 90% or greater. Alternatively, highly pure oligonucleotides can be obtained by a RP-HPLC “trityl off” method using an XTerra C18 column. The use of volatile triethylamine acetate buffer as an ion-pair for RP-HPLC eliminates the need for further desalting." @default.
- W2025116989 created "2016-06-24" @default.
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- W2025116989 date "2000-08-01" @default.
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- W2025116989 title "Purification of crude DNA oligonucleotides by solid-phase extraction and reversed-phase high-performance liquid chromatography" @default.
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- W2025116989 doi "https://doi.org/10.1016/s0021-9673(00)00521-5" @default.
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